MCPH1 inhibits Condensin II during interphase by regulating its SMC2-Kleisin interface

eLife

Lothar Schermelleh¹  Jonathan Godwin¹  Martin Houlard¹  Kim Nasmyth¹  Erin E Cutts²  Alessandro Vannini³  David Weisz⁴  Erez Lieberman Aiden⁴  Aviva Presser Aiden⁴  Muhammad S Shamim⁵ 

[1]Department of Biochemistry, University of Oxford, Oxford, United Kingdom

[2]Division of Structural Biology, The Institute of Cancer Research, London, United Kingdom

[3]Division of Structural Biology, The Institute of Cancer Research, London, United Kingdom

[4]Human Technopole, Milan, Italy

[5]The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States

[6]Center for Theoretical Biological Physics, Rice University, Houston, United States

[7]The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, United States

[8]Medical Scientist Training Program, Baylor College of Medicine, Department of Bioengineering, Rice University, Houston, United States

[9]Center for Theoretical Biological Physics, Rice University, Houston, United States

关键词: condensin;    chromosome;    microcephalin;    condensation;    cell cycle;    cohesin;    Human;    Mouse;   

DOI  :  10.7554/eLife.73348

来源: eLife Sciences Publications, Ltd

【 摘 要 】

Dramatic change in chromosomal DNA morphology between interphase and mitosis is a defining features of the eukaryotic cell cycle. Two types of enzymes, namely cohesin and condensin confer the topology of chromosomal DNA by extruding DNA loops. While condensin normally configures chromosomes exclusively during mitosis, cohesin does so during interphase. The processivity of cohesin’s loop extrusion during interphase is limited by a regulatory factor called WAPL, which induces cohesin to dissociate from chromosomes via a mechanism that requires dissociation of its kleisin from the neck of SMC3. We show here that a related mechanism may be responsible for blocking condensin II from acting during interphase. Cells derived from patients affected by microcephaly caused by mutations in the MCPH1 gene undergo premature chromosome condensation. We show that deletion of Mcph1 in mouse embryonic stem cells unleashes an activity of condensin II that triggers formation of compact chromosomes in G1 and G2 phases, accompanied by enhanced mixing of A and B chromatin compartments, and this occurs even in the absence of CDK1 activity. Crucially, inhibition of condensin II by MCPH1 depends on the binding of a short linear motif within MCPH1 to condensin II’s NCAPG2 subunit. MCPH1’s ability to block condensin II’s association with chromatin is abrogated by the fusion of SMC2 with NCAPH2, hence may work by a mechanism similar to cohesin. Remarkably, in the absence of both WAPL and MCPH1, cohesin and condensin II transform chromosomal DNAs of G2 cells into chromosomes with a solenoidal axis.

【 授权许可】

CC BY