Condensin I subunit Cap-G is essential for proper gene expression during the maturation of post-mitotic neurons

eLife

Tony D Southall¹  Pablo Araguas Rodriguez¹  Emma L Walmsley¹  Amira Hassan¹  Gabriel N Aughey¹  Stefan K Heidmann² 

[1] Department of Life Sciences, Imperial College London, London, United Kingdom;Lehrstuhl für Genetik, University of Bayreuth, Bayreuth, Germany;

关键词: condensin;    neuron;    development;    post-mitotic;    DamID;    D. melanogaster;   

DOI  :  10.7554/eLife.55159

来源: publisher

【 摘 要 】

Condensin complexes are essential for mitotic chromosome assembly and segregation during cell divisions, however, little is known about their functions in post-mitotic cells. Here we report a role for the condensin I subunit Cap-G in Drosophila neurons. We show that, despite not requiring condensin for mitotic chromosome compaction, post-mitotic neurons express Cap-G. Knockdown of Cap-G specifically in neurons (from their birth onwards) results in developmental arrest, behavioural defects, and dramatic gene expression changes, including reduced expression of a subset of neuronal genes and aberrant expression of genes that are not normally expressed in the developing brain. Knockdown of Cap-G in mature neurons results in similar phenotypes but to a lesser degree. Furthermore, we see dynamic binding of Cap-G at distinct loci in progenitor cells and differentiated neurons. Therefore, Cap-G is essential for proper gene expression in neurons and plays an important role during the early stages of neuronal development.

【 授权许可】

CC BY