The association between interferon lambda 3 and 4 gene single-nucleotide polymorphisms and the recovery of COVID-19 patients

Virology Journal

Jalal Mosayebi Amroabadi¹  Alireza Rahimpour²  Rahil Tarharoudi³  Abolfazl Fateh⁴  Seyed Davar Siadat⁴  Iraj Ahmadi⁵  Enayat Anvari⁵  Pooneh Rahimi⁶  Mohammadreza Aghasadeghi⁶ 

[1] Artificial Intelligence and Multi-Omics Center (AIMOC), Stavanger, Norway;Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran;Department of Molecular and Cellular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran;Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran;Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran;Department of Physiology, School of Medicine, Ilam University of Medical Science, Ilam, Iran;Hepatitis and AIDS Department, Pasteur Institute of Iran, Tehran, Iran;Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran;

关键词: SARS-CoV-2;    COVID-19;    Interferon lambda 3;    Interferon lambda 4;    Single-nucleotide polymorphisms;   

DOI  :  10.1186/s12985-021-01692-z

来源: Springer

【 摘 要 】

BackgroundThe recent pandemic caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has elevated several clinical and scientific questions. These include how host genetic factors influence the pathogenesis and disease susceptibility. Therefore, the aim of this study was to evaluate the impact of interferon lambda 3 and 4 (IFNL3/4) gene polymorphisms and clinical parameters on the resistance and susceptibility to coronavirus disease 2019 (COVID-19) infection.MethodsA total of 750 SARS-CoV-2 positive patients (375 survivors and 375 nonsurvivors) were included in this study. All single-nucleotide polymorphisms (SNPs) on IFNL3 (rs12979860, rs8099917, and rs12980275) and IFNL4 rs368234815 were genotyped by the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) method.ResultsIn this study, a higher viral load (low PCR Ct value) was shown in nonsurvivor patients. In survivor patients, the frequency of the favorable genotypes of IFNL3/4 SNPs (rs12979860 CC, rs12980275 AA, rs8099917 TT, and rs368234815 TT/TT) was significantly higher than in nonsurvivor patients. Multivariate logistic regression analysis has shown that a higher low-density lipoprotein (LDL), erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), and PCR Ct value, and lower 25-hydroxyvitamin D, and also IFNL3 rs12979860 TT, IFNL3 rs8099917 GG, IFNL3 rs12980275 GG, and IFNL4 rs368234815 ∆G/∆G genotypes were associated with the severity of COVID-19 infection.ConclusionsThe results of this study proved that the severity of COVID-19 infection was associated with clinical parameters and unfavorable genotypes of IFNL3/IFNL4 SNPs. Further studies in different parts of the world are needed to show the relationship between severity of COVID-19 infection and host genetic factors.

【 授权许可】

CC BY