Screening of CXC chemokines in the microenvironment of ovarian cancer and the biological function of CXCL10

World Journal of Surgical Oncology

Ji-Ao Ma¹  Chunjie Xiao¹  Xun Sheng¹  Weiyuan Li¹ 

[1] School of Medicine, Yunnan University, No.2, Cuihu North Road, 650091, Kunming, Yunnan Province, People’s Republic of China;

关键词: Ovarian cancer;    Chemokines;    Angiogenesis;    Immune infiltration;    Bioinformatics;   

DOI  :  10.1186/s12957-021-02440-x

来源: Springer

【 摘 要 】

BackgroundThis study aims to screen and identify the biological functions and prognostic value of CXC chemokines in ovarian cancer (OC) through bioinformatics and molecular biology methods, and to provide data support for the selection of biomarkers and prognostic analysis of OC.MethodsIn this study, GEO, ONCOMINE, GEPIA, cBioPortal, GeneMANIA, Metascape, STRING, TRRUST, and TIMER databases were used to study CXC chemokines. Angiogenesis and T cell killing assay were used to detect the effect of CXCL10 on tumor cell immunity and angiogenesis. Real-time quantitative PCR (qRT-PCR), immunoblotting, and ectopic tumor formation experiments were used to verify the effect of CXCL10 on ovarian cancer tumors.ResultsWe found that CXCL1, CXCL10, CXCL11, CXCL13, and CXCL14 were significantly upregulated in OC samples compared with normal tissues. Our data showed that there was a relationship between the expression of CXC chemokines and the infiltration of six types of immune cells significant correlation. In vitro assay confirmed that overexpression of CXCL10 could enhance the killing effect of T cells and inhibit angiogenesis. Further in vivo assay had shown that CXCL10 could affect the progression of ovarian cancer by increasing the expression of cytotoxic T cells and inhibiting angiogenesis.ConclusionIn conclusion, we hope that our data will provide new insights into the development of immunotherapy and the selection of prognostic markers for patients with OC.

【 授权许可】

CC BY