Cyanidin-3-glucoside suppresses the progression of lung adenocarcinoma by downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway

World Journal of Surgical Oncology

Xiuzhen Xu¹  Xiaojun Chen²  Weixia Zhang³ 

[1] Department of Emergency, Weifang Brain Hospital, 423 Dongfeng West Street, Weicheng District, 261021, Weifang, Shandong, P.R. China;Department of Emergency, Weifang Hospital of Traditional Chinese Medicine, 261041, Weifang, Shandong, P.R. China;Department of Urology, Weifang Hospital of Traditional Chinese Medicine, 261041, Weifang, Shandong, P.R. China;

关键词: LUAD;    C3G;    TP53I3;    Apoptosis;    Metastasis;    PI3K/AKT/mTOR pathway;   

DOI  :  10.1186/s12957-021-02339-7

来源: Springer

【 摘 要 】

BackgroundThe aim of this study is to unravel the role of Cyanidin-3-glucoside (C3G) and its potential mechanisms in lung adenocarcinoma (LUAD).MethodsThe cell clones, proliferation, apoptosis, migration, and invasion in H1299 and A549 cells were determined by colony formation assay, 5-ethynyl-20 deoxyuridine (EdU) assay, flow cytometry, and transwell assay, respectively. The expression of p53-induced gene 3 (TP53I3) was assessed and the prognostic values of TP53I3 in LUAD via the dataset from the Cancer Genome Atlas (TCGA). In addition, the mRNA and protein expressions were detected by quantitative real-time PCR (qRT-PCR) and western blot.ResultsC3G inhibited the proliferation, migration, and invasion of, and also promoted the apoptosis in H1299 and A549 cells. The database of TCGA showed TP53I3 was highly expressed in LUAD tissues and correlated with the poor prognosis of LUAD patients. Moreover, we also found that C3G inhibited the proliferation, migration and invasion, and promoted apoptosis in H1299 and A549 cells by downregulating TP53I3. Additionally, C3G could inhibit the activation of phosphatidylinositol 3′-kinase (PI3K)/protein kinase B (AKT)/mammalian target of rapamycin (mTOR) pathway in H1299 and A549 cells by downregulating TP53I3.ConclusionThis study demonstrated that C3G could inhibit the proliferation, migration and invasion, and also facilitate the apoptosis through downregulating TP53I3 and inhibiting PI3K/AKT/mTOR pathway in LUAD.

【 授权许可】

CC BY