Microfragmented adipose tissue is associated with improved ex vivo performance linked to HOXB7 and b-FGF expression

Stem Cell Research & Therapy

Giulia Casari¹  Massimo Dominici²  Elena Veronesi³  Filippo Selleri⁴  Andrea Giorgini⁴  Fabio Catani⁴  Elisabetta Manuela Foppiani⁵  Marta Starnoni⁶  Massimo Pinelli⁶  Giorgio De Santis⁶  Lucrezia Pacchioni⁶  Olivia Candini⁷  Elisa Resca⁸  Maria Serena Piccinno⁸  Tiziana Petrachi⁸ 

[1] Department of Medical and Surgical Sciences for Children and Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy;Rigenerand srl, Medolla, Modena, Italy;Department of Medical and Surgical Sciences for Children and Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy;Rigenerand srl, Medolla, Modena, Italy;Technopole Mario Veronesi, Mirandola, Modena, Italy;Department of Medical and Surgical Sciences for Children and Adults, University-Hospital of Modena and Reggio Emilia, Modena, Italy;Technopole Mario Veronesi, Mirandola, Modena, Italy;Department of Orthopaedic and Traumatology, University-Hospital of Modena and Reggio Emilia, Modena, Italy;Department of Pediatrics, Emory University, Atlanta, USA;Division of Plastic Surgery, Department of General Surgery and Surgical Specialties, University-Hospital of Modena and Reggio Emilia, Modena, Italy;Rigenerand srl, Medolla, Modena, Italy;Technopole Mario Veronesi, Mirandola, Modena, Italy;

关键词: Lipogems;    MSC;    Adipose tissue;    Osteoarthritis;    Microfragmentation;   

DOI  :  10.1186/s13287-021-02540-1

来源: Springer

【 摘 要 】

IntroductionAdipose tissue (AT) has become a source of mesenchymal stromal/stem cells (MSC) for regenerative medicine applications, in particular skeletal disorders. Several enzymatic or mechanical procedures have been proposed to process AT with the aim to isolate cells that can be locally implanted. How AT is processed may impact its properties. Thus, we compared AT processed by centrifugation (C-AT) to microfragmentation (MF-AT). Focusing on MF-AT, we subsequently assessed the impact of synovial fluid (SF) alone on both MF-AT and isolated AT-MSC to better understand their cartilage repair mechanisms.Materials and methodsMF-AT and C-AT from the same donors were compared by histology and qRT-PCR immediately after isolation or as ex vivo cultures using a micro-tissue pellet system. The in vitro impact of SF on MF-AT and AT-MSC was assessed by histological staining and molecular analysis.ResultsThe main AT histological features (i.e., increased extracellular matrix and cellularity) of the freshly isolated or ex vivo-cultured MF-AT persisted compared to C-AT, which rapidly deteriorated during culture. Based on our previous studies of HOX genes in MSC, we investigated the involvement of Homeobox Protein HOX-B7 (HOXB7) and its target basic Fibroblast Growth Factor (bFGF) in the molecular mechanism underlying the improved performance of MF-AT. Indeed, both these biomarkers were more prominent in freshly isolated MF-AT compared to C-AT. SF alone preserved the AT histological features of MF-AT, together with HOXB7 and bFGF expression. Increased cell performance was also observed in isolated AT-MSC after SF treatment concomitant with enhanced HOXB7 expression, although there was no apparent association with bFGF.ConclusionsOur findings show that MF has a positive effect on the maintenance of AT histology and may trigger the expression of trophic factors that improve tissue repair by processed AT.

【 授权许可】

CC BY