Gene expression of tendon markers in mesenchymal stromal cells derived from different sources

BMC Research Notes

Walter Brehm²  Annette B Ahrberg³  Felicitas Paebst²  Bastian Pfeiffer²  Sandra Heller¹  Claudia Gittel²  Janina Burk² 

[1]Tulane University, Department of Pathology and Laboratory Medicine, 1430 Tulane Avenue, New Orleans, Louisiana 70112, USA

[2]Large Animal Clinic for Surgery, Faculty of Veterinary Medicine, University of Leipzig, An den Tierkliniken 21, 04103 Leipzig, Germany

[3]Department of Orthopedics, Traumatology and Plastic Surgery, University of Leipzig, Liebigstrasse 20, 04103 Leipzig, Germany

关键词: Tenascin-C;    Scleraxis;    Decorin;    Collagen;    Umbilical cord;    Bone marrow;    Adipose tissue;    Tendon;    Mesenchymal stromal cell;    MSC;   

Others  :  1118377
DOI  :  10.1186/1756-0500-7-826

 received in 2014-10-25, accepted in 2014-11-12,  发布年份 2014

【 摘 要 】

Background

Multipotent mesenchymal stromal cells (MSC) can be recovered from a variety of tissues in the body. Yet, their functional properties were shown to vary depending on tissue origin. While MSC have emerged as a favoured cell type for tendon regenerative therapies, very little is known about the influence of the MSC source on their properties relevant to tendon regeneration.

The aim of this study was to assess and compare the expression of tendon extracellular matrix proteins and tendon differentiation markers in MSC derived from different sources as well as in native tendon tissue. MSC isolated from equine bone marrow, adipose tissue, umbilical cord tissue, umbilical cord blood and tendon tissue were characterized and then subjected to mRNA analysis by real-time polymerase chain reaction.

Results

MSC derived from adipose tissue displayed the highest expression of collagen 1A2, collagen 3A1 and decorin compared to MSC from all other sources and native tendon tissue (p < 0.01). Tenascin-C and scleraxis expressions were highest in MSC derived from cord blood compared to MSC derived from other sources, though both tenascin-C and scleraxis were expressed at significantly lower levels in all MSC compared to native tendon tissue (p < 0.01).

Conclusions

These findings demonstrate that the MSC source impacts the cell properties relevant to tendon regeneration. Adipose derived MSC might be superior regarding their potential to positively influence tendon matrix reorganization.

【 授权许可】

   
2014 Burk et al.; licensee BioMed Central Ltd.

【 图 表 】

Figure 1.

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