Adhesion of monocytes and endothelial cells isolated from the human aorta suppresses by miRNA-PEI particles

BMC Cardiovascular Disorders

Mohammad Najafi¹  Golnaz Esfandiari¹  Adeleh Poursaleh¹  Farnaz Sadegh Beigee² 

[1] Biochemistry Department, Microbial Biotechnology Research Center, Iran University of Medical Sciences, Tehran, Iran;Masih Daneshvari Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran;

关键词: Endothelial cell;    miR-125;    miR-495;    ICAM-1;    ICAM-2;    ITGB-2;    VCAM-1;   

DOI  :  10.1186/s12872-021-02203-2

来源: Springer

【 摘 要 】

BackgroundKnowledge of stenosis in coronary arteries requires an understanding of the cellular and molecular processes that occur throughout the leukocyte rolling process. In this study, the roles of miR-125a-5p and miR-495-3p were investigated on the adhesion of endothelial cells (ECs) isolated from the human aorta.MethodsHuman primary endothelial cells were obtained from the aorta of people who had died of brain death. Whole blood was used to isolate the monocytes. The miR-125 and miR-495 were predicted and transfected into ECs using Poly Ethylene Imine (PEI). The expression levels of adhesion molecules and monocyte recruitment were identified by the RT-qPCR technique and Leukocyte-Endothelial Adhesion Assay kit, respectively.ResultsThe ICAM-1, ICAM-2 and VCAM-1 expression levels decreased significantly in the miR-495/PEI-transfected ECs (P < 0.05) while in the miR-125/PEI-transfected ECs only the ICAM-2 and ITGB-2 expression levels decreased significantly (P < 0.05) as compared to the miR-synthetic/PEI-transfected ECs. Furthermore, the monocyte adhesion was decreased in the miR-125 and miR-mix/PEI-transfected ECs as compared to the miR-synthetic/PEI-transfected ECs (P = 0.01 and P = 0.04, respectively).ConclusionAccording to the findings, the efficient relations between miR-125 and adhesion molecules may be responsible for the inhibition of monocyte rolling.

【 授权许可】

CC BY