E‐selectin and ICAM‐1 are incorporated into detergent‐insoluble membrane domains following clustering in endothelial cells

FEBS Letters

Hoover, Richard L¹  Tilghman, Robert W¹ 

[1] Department of Pathology, Vanderbilt University School of Medicine, U4202 Medical Center North, 1161 21 Avenue South, Nashville, TN 37232, USA

关键词: Endothelial cell;    Lipid raft;    Tyrosine kinase;    Adhesion;    HUVEC;    human umbilical vein endothelial cell;    ICAM-1;    intercellular adhesion molecule-1;    TNFα;    tumor necrosis factor-α;    BCD;    β-cyclodextrin;   

DOI  :  10.1016/S0014-5793(02)03070-3

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

Here we present data supporting the role of lipid rafts in endothelial cells during leukocyte adhesion. Following adhesion of THP-1 cells or antibody-mediated clustering, both E-selectin and intercellular adhesion molecule-1 (ICAM-1) partitioned into the detergent-insoluble portion of the endothelial cellular lysate. Sucrose gradient centrifugation revealed the partitioning of clustered E-selectin and ICAM-1 with the low-density fraction where they co-fractionated with src family kinases, markers of lipid rafts. Depleting the plasma membrane of cholesterol inhibited clustering of adhesion molecules following their antibody-induced crosslinking and inhibited their association with src kinases. Thus, our data suggest that E-selectin and ICAM-1 associate with lipid rafts in human endothelial cells following leukocyte adhesion.

【 授权许可】

Unknown