Comparison of demineralized bone matrix and hydroxyapatite as carriers of Escherichia coli recombinant human BMP-2

Biomaterials Research

Guang-Bin Zheng¹  Jae Hyup Lee²  Shi-Huan Han³  Yuan-Zhe Jin⁴ 

[1]Department of Orthopaedics, Taizhou Hospial of Zhejiang Province, 317000, Linhai, Zhejiang, China

[2]Department of Orthopedic Surgery, College of Medicine, Seoul National University, 03080, Seoul, South Korea

[3]Department of Orthopedic Surgery, SMG-SNU Boramae Medical Center, 39 Boramae Gil, Dongjak-Gu, 156-707, Seoul, South Korea

[4]Department of Orthopaedic Surgery, Seoul National University, College of Medicine, Institute of Medical and Biological Engineering, Seoul National University Medical Research Centre, SMG-SNU Boramae Medical Centre, Boramae-ro 5-gil 20, Dongjak-gu, 07061, Seoul, South Korea

[5]Department of Orthopedic Surgery, College of Medicine, Seoul National University, 03080, Seoul, South Korea

[6]Department of Orthopedic Surgery, YanBian University Hospital, 133000, Yanji, Jilin Province, China

[7]Department of Orthopedic Surgery, College of Medicine, Seoul National University, 03080, Seoul, South Korea

[8]The First Hospital of Jilin University, 130021, Changchun City, China

关键词: Bone morphogenetic protein-2;    Demineralized bone matrix;    Hydroxyapatite;    Carrier;    Bone regeneration;   

DOI  :  10.1186/s40824-021-00225-7

来源: Springer

【 摘 要 】

BackgroundAutograft has been widely used in various orthopedic and dental surgery for its superior osteogenicity, osteoinductivity and osteoconductivity. But the available volume of the autograft is limited and the efficacy of it is highly affected by the condition of the patients. Therefore, growth factors such as Escherichia coli bone morphogenetic protein-2 (ErhBMP-2) has been widely used in some countries and regions with various carriers that could affect the effects of the growth factors. Demineralized bone matrix (DBM) has been widely used as a bone graft substitute and growth factor carrier, but its effect as a carrier of ErhBMP-2 was less investigated.Materials and methodsRat calvaria defect model was used in this study. We implanted ErhBMP-2 with DBM or hydroxyapatite (HA) as a carrier in 8 mm calvaria defect and compared their bone regeneration effect in 4th week and 8th week after implantation with micro-CT and histology. The data was analyzed with one-way ANOVA method with Bonferroni post-hoc analysis.ResultThe group with DBM as the carrier showed significantly higher bone volume and bone thickness than the groups with HA as the carrier in both weeks. And the histology sections showed less adipose tissue formed in the groups with DBM as the carrier.ConclusionDBM could be a better carrier for ErhBMP-2 than HA.

【 授权许可】

CC BY