Characterization of Adult Stem/Progenitor Cell Populations from Bone Marrow in a Three-Dimensional Collagen Gel Culture System

Cell Transplantation

Article

Silvia Claros¹  José A. Andrades¹  José Becerra²  Enrique Guerado³  Encarnación Cruz³  Noela Rodríguez-Losada⁴ 

[1] Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Málaga, Málaga, Spain;Department of Cell Biology, Genetics and Physiology, Faculty of Sciences, Networking Biomedical Research Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), University of Málaga, Málaga, Spain;University of Málaga, BIONAND, Málaga, Spain;Hospital Costa del Sol, Division of Orthopaedic Surgery and Traumatology, Marbella, Spain;Research Laboratory, Heart Unit, Virgen de la Victoria University Hospital, Campus de Teatinos, Málaga, Spain;

关键词: Stem/progenitor cells;    Three-dimensional (3D) culture;    Transforming growth factor-β1 (TGF-β1);    Osteogenesis;    Demineralized bone matrix;   

DOI  :  10.3727/096368912X636939

 received in 2011-02-24, accepted in 2011-10-22,  发布年份 2012

来源: Sage Journals

【 摘 要 】

Stem cell transplantation therapy using mesenchymal stem cells (MSCs) is considered a useful strategy. Although MSCs are commonly isolated by exploiting their plastic adherence, several studies have suggested that there are other populations of stem and/or osteoprogenitor cells that are removed from primary culture during media replacement. Therefore, we developed a three-dimensional (3D) culture system in which adherent and nonadherent stem cells are selected and expanded. Here, we described the characterization of 3D culture-derived cell populations in vitro and the capacity of these cells to differentiate into bone and/or cartilage tissue when placed inside of demineralized bone matrix (DBM) cylinders, implanted subcutaneously into the backs of rat for 2, 4, and 8 weeks. Our results demonstrates that 3D culture cells were a heterogeneous population of uncommitted cells that express pluripotent-, hematopoietic-, mesenchymal-, and endothelial-specific markers in vitro and can undergo osteogenic differentiation in vivo.

【 授权许可】

Unknown   
© 2012 Cognizant Comm. Corp.

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