Combination of modified carbapenem inactivation method (mCIM) and EDTA-CIM (eCIM) for phenotypic detection of carbapenemase-producing Enterobacteriaceae

BMC Microbiology

article

Tsai, Ya-Min¹  Wang, Shining²  Chiu, Hui-Chuan¹  Kao, Cheng-Yen²  Wen, Li-Li¹ 

[1] Department of Clinical Laboratory, En Chu Kong Hospital;Institute of Microbiology and Immunology, School of Life Science, National Yang-Ming University

关键词: Carbapenemase;    Enterobacteriaceae;    eCIM;    mCIM;    Phenotypic detection;   

DOI  :  10.1186/s12866-020-02010-3

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Carbapenemase-resistant Enterobacteriaceae (CRE) cause many serious infections resulting in increasing treatment cost, prolonged hospitalization, and mortality rate. Reduced expression and/or mutations of porins and the presence of carbapenemase promote Enterobacteriaceae survival under carbapenem treatments. Development of accurate methods for the detection of antimicrobial resistance is required not only for therapy but also to monitor the spread of resistant bacteria or resistance genes throughout the hospital and community. In this study, we aimed to evaluate the phenotypic methods, Modified Hodge test (MHT), modified carbapenem inactivation method (mCIM), and EDTA-CIM (eCIM) for the detection of carbapenemase-producing Enterobacteriaceae (CPE). The results showed that mCIM had a sensitivity of 100% and a specificity of 100%, whereas the MHT had a sensitivity of 84.8% and a specificity of 97.8% for the 195 CRE isolates tested (105 CPE and 90 non-CPE isolates). The sensitivity of the mCIM/eCIM to detect metallo-carbapenemases in this study was 89.3% and the specificity was 98.7% as compared to the genotypic PCR detection. These findings indicate that the mCIM combined with eCIM is useful for detecting and distinguishing different types of carbapenemase in Enterobacteriaceae.

【 授权许可】

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