期刊论文详细信息
BMC Microbiology
Enterohaemorrhagic Escherichia coli activates nitrate respiration to benefit from the inflammatory response for initiation of microcolony-formation
article
Nada, Risa1  Ebihara, Shinya1  Yen, Hilo1  Tobe, Toru1 
[1]Department of Clinical Laboratory and Biomedical Sciences, Osaka University Graduate School of Medicine
关键词: T3SS;    Nitric oxide;    Adherence;    Colony formation;    Anaerobic conditions;   
DOI  :  10.1186/s12866-020-01946-w
学科分类:放射科、核医学、医学影像
来源: BioMed Central
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【 摘 要 】
For successful colonization, enterohaemorrhagic Escherichia coli (EHEC) injects virulence factors, called effectors, into target cells through the type three secretion system (T3SS), which is composed of a needle and basal body. Under anaerobic conditions, the T3SS machinery remains immature and does not have a needle structure. However, activation of nitrate respiration enhances the completion of the T3SS machinery. Because nitric oxide released by the host inflammatory response increases nitrate concentration, we sought to determine the effect of the inflammatory response on initiation of EHEC microcolony-formation. The colony-forming capacity was increased in accordance with the increase of nitrate in the medium. The addition of the nitric oxide-producing agent NOR-4 also enhanced the adherence capacity, which was dependent on nitrate reductase encoded by the narGHJI genes. Culture supernatant of epithelial cells, which was stimulated by a cytokine mixture, enhanced the colony-forming capacity of wild-type EHEC but not of the narGHJI mutant. Finally, colony formation by wild-type EHEC on epithelial cells, which were preincubated with heat-killed bacteria, was higher than the narGHJI mutant, and this effect was abolished by aminoguanidine hydrochloride, which is an iNOS (inducible nitric oxide synthase) inhibitor. These results indicate that the inflammatory response enhances EHEC adherence by increasing nitrate concentration.
【 授权许可】

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