Study of budding yeast colony formation and its characterizations by using circular granular cell

13th South-East Asian Congress of Medical Physics 2015

物理学;医药卫生

Aprianti, D.^1 ; Haryanto, F.^2 ; Purqon, A.^2 ; Khotimah, S.N.^2 ; Viridi, S.^2

Department of Computational Science, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jalan Ganesa 10, Bandung

40132, Indonesia^1

Department of Physics, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Jalan Ganesa 10, Bandung

40132, Indonesia^2

关键词: Cohesion strength;    Colony expansion;    Colony formation;    Effective therapy;    Growth substrates;    Irregular shape;    Minimum distance;    Solid substrates;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/694/1/012079/pdf
DOI  :  10.1088/1742-6596/694/1/012079

学科分类：卫生学

来源: IOP

【 摘 要 】

Budding yeast can exhibit colony formation in solid substrate. The colony of pathogenic budding yeast can colonize various surfaces of the human body and medical devices. Furthermore, it can form biofilm that resists drug effective therapy. The formation of the colony is affected by the interaction between cells and with its growth media. The cell budding pattern holds an important role in colony expansion. To study this colony growth, the molecular dynamic method was chosen to simulate the interaction between budding yeast cells. Every cell was modelled by circular granular cells, which can grow and produce buds. Cohesion force, contact force, and Stokes force govern this model to mimic the interaction between cells and with the growth substrate. Characterization was determined by the maximum (Lmax) and minimum (Lmin) distances between two cells within the colony and whether two lines that connect the two cells in the maximum and minimum distances intersect each other. Therefore, it can be recognized the colony shape in circular, oval, and irregular shapes. Simulation resulted that colony formation are mostly in oval shape with little branch. It also shows that greater cohesion strength obtains more compact colony formation.