Effects of iron on the growth, biofilm formation and virulence of Klebsiella pneumoniae causing liver abscess

BMC Microbiology

article

Chen, Tao¹  Dong, Guofeng²  Zhang, Siqin¹  Zhang, Xiucai¹  Zhao, Yajie³  Cao, Jianming³  Zhou, Tieli¹  Wu, Qing¹ 

[1] Department of Clinical Laboratory, The First Affiliated Hospital of Wenzhou Medical University;Xiangyang NO.1. People Hospital, Affiliated Hospital of Hubei University of Medicine;School of Laboratory Medicine and Life Sciences, Wenzhou Medical University

关键词: Iron;    Klebsiella pneumoniae;    Growth;    Biofilm;    Virulence;   

DOI  :  10.1186/s12866-020-01727-5

学科分类：放射科、核医学、医学影像

来源: BioMed Central

【 摘 要 】

Klebsiella pneumoniae is considered the most clinically relevant species of Enterobacteriaceae, known to cause severe infections including liver abscesses. To the best of our knowledge, a large proportion of iron in the human body is accumulated and stored in the liver. We hypothesize that increased iron availability is an important factor driving liver abscess formation and we therefore aim to understand the effects of iron on K. pneumoniae causing liver abscesses. All tested K. pneumoniae clinical isolates, including those isolated from liver abscesses and other abdominal invasive infection sites, grew optimally when cultured in LB broth supplemented with 50 μM iron and exhibited the strongest biofilm formation ability under those conditions. Decreased growth and biofilm formation ability were observed in all tested strains when cultured with an iron chelator (P < 0.05). The infection model of G. mellonella larvae indicated the virulence of liver abscess-causing K. pneumoniae (2/3) cultured in LB broth with additional iron was significantly higher than those under iron-restricted conditions (P < 0.05). The relative expression levels of the four siderophore genes (iucB, iroB, irp1, entB) in K. pneumoniae strains isolated from liver abscesses cultured with additional iron were lower than those under iron-restricted conditions (P < 0.05). It is suggested by our research that iron in the environment can promote growth, biofilm formation and enhance virulence of K. pneumoniae causing liver abscesses. A lower expression of siderophore genes correlates with increased virulence of liver abscess-causing K. pneumoniae. Further deeper evaluation of these phenomena is warranted.

【 授权许可】

CC BY|CC0