Investigation of Staphylococcus aureus positive for Staphylococcal enterotoxin S and T genes

The Journal of Veterinary Medical Science

article

Yusuke SATO’O¹  Katsuhiko OMOE²  Yasuko AIKAWA²  Mayuko KANO²  Hisaya K. ONO²  Dong-Liang HU⁶  Akio NAKANE⁵  Motoyuki SUGAI¹ 

[1] Department of Bacteriology, Hiroshima University Graduate School of Biomedical & Health Sciences;Department of Veterinary Medicine, Faculty of Agriculture, Iwate University;The United Graduate School of Veterinary Sciences, Gifu University;Present address: Division of Bacteriology, Department of infection and immunity, School of Medicine, Jichi Medical University;Department of Microbiology and Immunology, Hirosaki University Graduate School of Medicine;Laboratory of Zoonoses, Kitasato University School of Veterinary Medicine;Present address: Antimicrobial Resistance Research Center, National Institute of Infectious Diseases (NIID)

关键词: plasmid;    Staphylococcus aureus;    staphylococcal enterotoxin;    staphylococcal food poisoning;   

DOI  :  10.1292/jvms.20-0662

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Staphylococcus aureus produces staphylococcal enterotoxins (SEs) and causes food poisoning. It is known that almost all SE-encoding genes are present on various types of mobile genetic elements and can mobilize among S. aureus populations. Further, plasmids comprise one of SE gene carriers. Previously, we reported novel SEs, SES and SET, harbored by the plasmid pF5 from Fukuoka5. In the present study, we analyzed the distribution of these SEs in various S. aureus isolates in Japan. We used 526 S. aureus strains and found 311 strains positive for at least one SE/SE-like toxin gene, but only two strains (Fukuoka5 and Hiroshima3) were positive for ses and set among the specimens. We analyzed two plasmids (pF5 and pH3) from these strains and found that they were different. Whereas these plasmids partially shared similar sequences involved in the ser / selj / set / ses gene cluster, other sequences were different. A comparison of these plasmids with those deposited in the NCBI database revealed that only one plasmid had the ser / selj / set / ses cluster with a stop mutation in set similar to that in pH3. In addition, the chromosomes of Fukuoka5 and Hiroshima3, positive for ses and set , were classified into different genotypes. Despite the low rate of gene positivity for these SEs, it is suggested that there is diversity in plasmids and strains carrying these two SEs. Consequently, regarding the entire feature of SE prevalence, we improved the multiplex PCR detection method for the SE superfamily to obtain further insight.

【 授权许可】

Unknown