Prevalence and Characterization of Staphylococcus aureus and Enterotoxigenic Staphylococcus aureus in Retail Raw Chicken Meat Throughout Japan

Journal of Veterinary Medical Science

Eri SATO³  Tetsurou INAMOTO¹  Kosuke MATSUMURA³  Chisa NAKANO³  Kosuke FUJIO³  Satoru KITAI³  Akira SHIMIZU³  Junichi KAWANO³  Ryo YASUDA³  Hiroshi KITAGAWA² 

[1] Hyogo Prefectural Awaji Inspection Center;Department of Bioresource and Agrobioscience, Graduate School of Science and Technology, Kobe University;Department of Microbiology and Immunology, Faculty of Agriculture, Kobe University

关键词: biotype;    coagulase type;    pulsed-field gel electrophoresis;    Staphylococcus aureus;    staphylococcal enterotoxin;   

DOI  :  10.1292/jvms.67.269

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

References(28)Cited-By(26)A total of 444 samples of raw chicken meat (thighs, breasts, wings, livers, gizzards, hearts and ovaries) that retailed at 145 different supermarkets in 47 prefectures in Japan were examined for contamination with Staphylococcus aureus in association with its enterotoxigenicity. S. aureus was isolated from 292 (65.8%) of the samples, and from 131 of the 145 supermarkets. There was no significant difference in the detection rate of S. aureus according to the type of meat examined. About 80% of 714 isolates belonged to the poultry (57.1%) and human biotypes (22.1%). Seventy-eight (21.7%) of 360 isolates were enterotoxigenic and isolated from 78 samples in 53 supermarkets in 31 prefectures. Staphylococcal enterotoxins (SEs) produced were SEB (50 isolates), SEA (14), SEC (8), SED (2), SEA+SEB (2), and SEA+SEC (2). Most of the enterotoxigenic isolates belonged to the human and poultry biotypes, coagulase type VII, VIII or IV, and were lysed by phages of group III. Identical SE types, biotypes, coagulase types and pulsed-field gel electrophoresis (PFGE) patterns were shown in isolates from different types of meat at the same supermarket and from samples taken from different supermarkets in the same prefectures or in isolates from samples obtained from several different prefectures. Among the 50 SEB-producing isolates, 27 yielded three similar PFGE patterns that differed by only a few fragments, suggesting that they were closely related genetically. The three patterns were found in isolates of samples that retailed at 17 supermarkets in 11 prefectures, indicating that they may be disseminated among raw chicken meat in Japan.

【 授权许可】

Unknown