Comparing recombinant MPB70/SahH and native 20-kDa protein for detecting bovine tuberculosis using ELISA

The Journal of Veterinary Medical Science

article

Yun Sang CHO¹  Sang Eun LEE¹  Jong-Tae WOO²  Jinsik OH³  Hwan Won CHOI⁴  Jin Hyeok KWON¹  Jeong-Tae KIM²  Gunwoo HA³  Sukchan JUNG¹ 

[1] Bacterial Disease Division, Department of Animal and Plant Health Research;Gyeonggi Province Veterinary Service Center;Ltd.;ChoongAng Vaccine Laboratory

关键词: Mycobacterium bovis;    receiver operating curve;    sensitivity;    serological diagnosis;    specificity;   

DOI  :  10.1292/jvms.20-0422

学科分类：兽医学

来源: Japanese Society of Veterinary Science

【 摘 要 】

Bovine tuberculosis (bTB) is a zoonosis caused by Mycobacterium bovis . Test-and-cull protocols and gross pathological examinations of abattoir animals as well as milk pasteurization have been implemented to prevent the spread of tuberculosis from animals to humans worldwide. Despite the importance of precise and rapid diagnostic tests, conventional methods including intradermal skin tests and γ-interferon assays are limited by the high rate of false-negative results for cattle in the late infectious stage and due to laborious and time-consuming procedures. Therefore, antibody detection methods such as enzyme-linked immunosorbent assay (ELISA) are urgently needed to supplement the established approaches and expand the diagnostic window. This study was conducted to develop a bTB ELISA by evaluating recombinant and native proteins and various assay parameters. We produced recombinant MPB70 and SahH (M70S) and a native 20-kDa protein (20K) and optimized the ELISA protocol. The 20K ELISA showed 94.4% sensitivity and 98.2% specificity with an optimal sample-to-positive ratio cut-off of 0.531. The sensitivity and specificity of M70S ELISA were 94.4% and 97.3%, respectively, with an optimal sample-to-negative ratio cut-off of 1.696. Both assays showed acceptable diagnostic efficiency and could be used for bTB diagnosis in combination with established methods for herd screening and to expand the diagnostic window.

【 授权许可】

Unknown