Transcriptomic changes in peripheral blood mononuclear cells with weight loss: systematic literature review and primary data synthesis

Genes & Nutrition

Susan Coort¹  Melissa C. Southey²  Kaitlin Day³  Aimee L. Dordevic³  Chiara Murgia⁴  Helen Truby⁵ 

[1] Department of Bioinformatics-BiGCaT, NUTRIM School of Nutrition and Translational Research in Metabolism, Maastricht University, Maastricht, The Netherlands;Department of Clinical Pathology, Melbourne Medical School, The University of Melbourne, Melbourne, Victoria, Australia;Cancer Epidemiology Division, Cancer Council Victoria, Melbourne, Victoria, Australia;Precision Medicine, School of Clinical Sciences at Monash Health, Monash University, Clayton, Victoria, Australia;Department of Nutrition, Dietetics and Food, Monash University, Level 1, 264 Ferntree Gully Road, 3168, Notting Hill, Victoria, Australia;School of Agriculture and Food, The University of Melbourne, Melbourne, Australia;School of Human Movement and Nutrition Sciences, University of Queensland, Brisbane, Australia;

关键词: Transcriptomics;    Obesity;    Weight loss intervention;    Overweight;    Gene expression;   

DOI  :  10.1186/s12263-021-00692-6

来源: Springer

【 摘 要 】

Background and objectivesPeripheral blood mononuclear cells (PBMCs) have shown promise as a tissue sensitive to subtle and possibly systemic transcriptomic changes, and as such may be useful in identifying responses to weight loss interventions. The primary aim was to comprehensively evaluate the transcriptomic changes that may occur during weight loss and to determine if there is a consistent response across intervention types in human populations of all ages.MethodsIncluded studies were randomised control trials or cohort studies that administered an intervention primarily designed to decrease weight in any overweight or obese human population. A systematic search of the literature was conducted to obtain studies and gene expression databases were interrogated to locate corresponding transcriptomic datasets. Datasets were normalised using the ArrayAnalysis online tool and differential gene expression was determined using the limma package in R. Over-represented pathways were explored using the PathVisio software. Heatmaps and hierarchical clustering were utilised to visualise gene expression.ResultsSeven papers met the inclusion criteria, five of which had raw gene expression data available. Of these, three could be grouped into high responders (HR, ≥ 5% body weight loss) and low responders (LR). No genes were consistently differentially expressed between high and low responders across studies. Adolescents had the largest transcriptomic response to weight loss followed by adults who underwent bariatric surgery. Seven pathways were altered in two out of four studies following the intervention and the pathway ‘cytoplasmic ribosomal proteins’ (WikiPathways: WP477) was altered between HR and LR at baseline in the two datasets with both groups. Pathways related to ‘toll-like receptor signalling’ were altered in HR response to the weight loss intervention in two out of three datasets.ConclusionsTranscriptomic changes in PBMCs do occur in response to weight change. Transparent and standardised data reporting is needed to realise the potential of transcriptomics for investigating phenotypic features.Registration numberPROSPERO: CRD42019106582

【 授权许可】

CC BY