Association of endothelial nitric oxide synthase gene variants with preeclampsia

Reproductive Health

Tayyaba Afsar¹  Dara Al-disi¹  Mahmoud Abulmeaty¹  Suhail Razak¹  Ali Almajwal¹  Abdulaziz Abdullah Al Khuraif²  Mohammed Arshad²  Sarwat Jahan³  Ghazala Shaheen³  Asmat Ullah⁴  Nousheen Bibi⁵  Rani Faryal⁶ 

[1]College of Applied Medical Sciences, Community Health Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia

[2]Dental Biomaterials Research Chair, Dental Health Department, College of Applied Medical Sciences, King Saud University, Riyadh, Kingdom of Saudi Arabia

[3]Department of Animal Sciences, Faculty of Biological Sciences, Quaid-I-Azam University, 45320, Islamabad, Pakistan

[4]Department of Biochemistry, Faculty of Biological Sciences, Quaid-I-Azam University, 45320, Islamabad, Pakistan

[5]Department of Bioinformatics, Shaheed Benazir Bhutto Women University, Peshawar, Pakistan

[6]Department of Microbiology, Faculty of Biological Sciences, Quaid-I-Azam University, 45320, Islamabad, Pakistan

关键词: Preeclampsia;    Endothelial nitric oxide synthase gene;    Nitric oxide;    Variants;   

DOI  :  10.1186/s12978-021-01213-9

来源: Springer

【 摘 要 】

BackgroundPreeclampsia (PE) is a complex pregnancy hypertensive disorder with multifaceted etiology. The endothelial nitric oxide synthase (eNOS) gene and nitric oxide (NO) levels has been reported to be associated with PE predisposition in various populations. Therefore, present study was designed to investigate the role of NO levels and eNOS gene variants in preeclamptic women in Pakistan.MethodsA total of 600 women were evaluated, 188 of PE with mild features, 112 of PE with severe features and 300 normotensive pregnant women. NO levels were detected by Greiss reaction method and genotyping following sequencing was conducted for eNOS gene variants. Further insilico studies were performed to get insights into the structural and functional impact of identifies mutation on eNOS protein as well as on protein regulation.ResultsReduced concentrations of NO were reported in all PE groups (p < 0.05) as compared to controls. The frequency of c.894 T (p.298Asp) and g.-786C alleles were significantly associated with PE. In addition, novel homozygous variant g.2051G > A was also significantly associated with PE when compared to normotensive women. Dynamic simulation studies revealed that Glu298Asp mutation destabilize the protein molecule and decrease the overall stability of eNOS protein. Molecular docking analysis of mutant promoter with transcription factors STAT3 and STAT6 proposed changes in protein regulation upon these reported mutations in upstream region of the gene.ConclusionConsidering the results of current study, the functional alterations induced by these variants may influence the bioavailability of NO and represents a genetic risk factor for increased susceptibility to PE. However, large studies or meta-analysis are necessary to validate these findings.

【 授权许可】

CC BY