Cross-sectional study and genotyping of rotavirus-A infections in ruminants in Kuwait

BMC Veterinary Research

Qais A. H. Majeed¹  Nadra-Elwgoud M. I. Abdou²  Michael D. Bowen³  Slavica Mijatovic-Rustempasic³  Ashraf A. Saad⁴  Attia Samy⁵ 

[1] Department of Science, College of Basic Education, PAAET, 23167,, Aridyia, Farwanyia, Kuwait;Early Warning Center for Transboundary Animal Diseases-Gulf Cooperation Council, PAAFR, P.O. box 21422, 1307 Safat,, Rabyia, Farwanyia, Kuwait;Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt;Viral Gastroenteritis Branch, Division of Viral Diseases, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 30329, Atlanta, GA, USA;Virology lab., Veterinary Laboratories, PAAFR, 1307 Safat,, Rabyia, Farwanyia, Kuwait;Department of Virology, Animal Health Research Institute, 12618, Dokki, Giza, Egypt;Virology lab., Veterinary Laboratories, PAAFR, 1307 Safat,, Rabyia, Farwanyia, Kuwait;Department of Virology, Faculty of Veterinary Medicine, Cairo University, 12211, Giza, Egypt;

关键词: RVA;    Immunochromatography;    ELISA;    RT-qPCR assay;    G10P[11];    Cattle;    Sheep;    Goats;   

DOI  :  10.1186/s12917-021-02944-4

来源: Springer

【 摘 要 】

BackgroundGroup A rotaviruses (RVA) are zoonotic pathogens responsible for acute enteritis in human and neonatal ruminants. This research aimed to determine the prevalence of RVA in ruminants (cattle, sheep, and goats) and investigate the circulating RVA genotypes in these animals in Kuwait. We conducted a cross-sectional study to detect RVA in ruminants, using an immunochromatography test (IC), direct sandwich ELISA test, and real-time RT-PCR (RT-qPCR) assay using fecal samples.ResultsA total of 400 cattle, 334 sheep, and 222 goats were examined. The prevalence of RVA was 5.3, 1.2, and 2.3%, respectively, using IC. The ELISA test detected RVA from 4.3% of cattle, 0.9% of sheep, and 1.8% of goats. There was a significant association between the occurrence of diarrhea and the presence of RVA in bovine fecal samples (p-value = 0.0022), while no statistical association between diarrhea and the presence of RVA in fecal samples of sheep and goats was observed (p-value = 0.7250; p-value = 0.4499, respectively). Twenty-three of the IC-positive samples (17 from cattle, two from sheep, and four from goats) were tested using a RT-qPCR RVA detection assay targeting the NSP3 gene. The results showed that 21 of 23 IC-positive samples tested positive by RT-qPCR. Detection of RVA genotypes revealed that G10P[11] was the predominant strain in cattle (58.8%), followed by G8P[1] (11.7%). One sheep sample was genotyped as G8P[1]. In addition, G6P[1] and G6P[14] were detected in goat samples.ConclusionThe present study revealed that the IC was more sensitive in detecting RVA antigen in fecal samples than the ELISA test. A higher occurrence of RVA infection was observed in cattle than in sheep and goats. This study suggests that RVA might be a risk factor of diarrhea in bovine calves less than 2 weeks old. This research also demonstrates the circulation of RVA in sheep and goat populations in Kuwait. Finally, the G10P[11] RVA genotype was the most prevalent genotype identified from cattle samples.

【 授权许可】

CC BY