Data-independent acquisition mass spectrometry quantitative proteomic analysis reveals that skin aging-related proteins differ between men and women

Biocell

article

HUI ZHANG¹  XUEJUN ZHANG¹  LIANGDAN SUN¹  SEN YANG¹  SHIRUI CHEN¹  MENGTING LIU¹  YAOCHI WANG¹  CONG XIN¹  JING MA¹  XIAODONG ZHENG¹  YUANDI HUANG¹  BO ZHANG² 

[1] Department of Dermatology, No. 1 Hospital, Anhui Medical University;Anhui Ferry Dermotological Institute;Institute of Dermatology, Anhui Medical University;Department of Oncology, The Second Affiliated Hospital of Anhui Medical University

关键词: Epidermal protein;    Mass spectrometer;    Skin barrier;    Gender-related skin aging;    Proteome;   

DOI  :  10.32604/biocell.2021.016524

学科分类：仪器

来源: Biocell

【 摘 要 】

The skin is the largest organ of the human body, and its aging is visible to the naked eye. The aging rate of men and women is slightly different. This study compared the protein expression of skin samples on the curved forearms of 11 healthy women and 9 healthy men. Quantitative proteomics analysis found that the expression of epidermal proteins in men and women of the same age group was different. Compared with female skin, in male skin, 20 proteins were upregulated, and 7 proteins were downregulated. These data suggest that men and women have differences in the speed of skin aging. For the first time in this experiment, a non-invasive mass spectrometer was used to detect 27 different-related epidermal proteins between men and women. Compared with women, among the 20 epidermal proteins upregulated in men, their functions can be classified into antioxidants, epidermal lipid metabolism, signal transduction, membrane transport, and cell biological processes; the 7 downregulated proteins are involved in a variety of biological processes and inflammatory reactions. Our experiments have discovered epidermal proteins related to the differences between men and women, enriching the library of epidermal differential proteins between men and women and enriching the mechanism of skin aging between men and women from the perspective of epidermal differential proteins.

【 授权许可】

CC BY