Assessment of antibiosis potential of Bacillus sp. against the soil-borne fungal pathogen Sclerotium rolfsii Sacc. ( Athelia rolfsii (Curzi) Tu & Kimbrough)

Egyptian Journal of Biological Pest Control

article

Kumari, Puja¹  Bishnoi, Santosh Kumar²  Chandra, Sheela¹ 

[1] Department of Bio-Engineering, Birla Institute of Technology;ICAR Indian Institute of Wheat and Barley Research

关键词: Bacillus species;    Biocontrol;    Antagonistic activity;    Sclerotium rolfsii;   

DOI  :  10.1186/s41938-020-00350-w

学科分类：化学工程（综合）

来源: Springer

【 摘 要 】

This study aimed to investigate the rhizosphere bacterial isolates’ antagonistic property against the soil-borne fungal phytopathogen, Sclerotium rolfsii Sacc. (Athelia rolfsii (Curzi) Tu & Kimbrough). The chemical control of the disease caused by S. rolfsii is economically and environmentally unsustainable, and therefore, a bio-control agent in the form of rhizospheric bacteria is gaining importance. Five rhizospheric Bacillus species viz. B. subtilis subsp. Subtilis str.168 (accession no. MH283878), B. siamensis strain PDA 10 (accession no. MH283879), B. amyloliquefaciens strain 1034 (accession no. MH283880), B. velezensis strain FZB42165 (accession no. MH283881), and B. atrophaeus strain NBRC 15539 (accession no. MH283882) were assessed for their antagonistic potential against S. rolfsii based on 3 different screening methods. Among these, 100% fungal growth inhibition by all 5 Bacillus spp. was observed in the novel ring method, whereas in the dual culture method, the maximum growth inhibition was (58%) exhibited by the strain NBRC 15539 of B. atrophaeus. The antagonistic activity showed by the modified dual culture method was also relatively high, and the highest activity (93.7%) was shown by the strain NBRC 15539 of B. atrophaeus. Besides, the Bacillus sp. was also evaluated for their plant growth-promoting attributes and other properties such as the production of siderophore, HCN, amylase, protease, lipase, and ammonia, including their assessment for chitinase and cellulase activity. The study provided empirical evidence of Bacillus sp. antagonistic potential against S. rolfsii and should be of contributive value in developing a biocontrol agent for this highly important crop fungal pathogen.

【 授权许可】

CC BY