The role of pulmonary ORCC and CLC-2 channels in the response to oxidative stress

Molecular & Cellular Toxicology

article

Canella, Rita¹  Benedusi, Mascia¹  Martini, Marta¹  Guiotto, Anna¹  Cervellati, Franco¹  Valacchi, Giuseppe¹ 

[1] Department of Neuroscience and Rehabilitation, University of Ferrara;Animal Science Department, Plants for Human Health Institute, NC State University;Department of Food and Nutrition, Kyung Hee University

关键词: Human lung epithelial cells;    Chloride channels;    Ozone;    Oxidative stress;    Patch clamp technique;   

DOI  :  10.1007/s13273-021-00137-6

来源: Korean Society of Toxicogenomics and Toxicoproteomics

【 摘 要 】

Background Exposure of human lung epithelial cells to the oxidant pollutant ozone (O3) alters cell Cl− currents inducing an outward rectifier effect. Among the various Cl− channels, ClC-2 and ORCC seemed to be involved in this response. Objectives To identify the channel related to O3 induced current changes. Results Down regulating the expression of ORCC and ClC-2 genes and analyzing the membrane current show that the enhancement of the current disappeared when ORCC was silenced. The contribution of ORCC and ClC-2 channels in control and O3 treated cells was obtained by a mathematical approach. Conclusion We suggest that O3 activates ORCC channels and slightly inhibited ClC-2 channels in the negative voltage range. These findings open the possibility of identifying the biomolecular changes induced by O3 allowing a possible pharmacological intervention towards chloride current due to oxidative stress.

【 授权许可】

Unknown