Macromolecular carbonyls in human stratum corneum: a biomarker for environmental oxidant exposure?

FEBS Letters

Packer, Lester²  Yan, Liang-Jun²  Cross, Carroll E¹  Re, Roberta²  Traber, Maret G²  Espuno, Nathalie²  Thiele, Jens J² 

[1]Division of Pulmonary-Critical Care Medicine, Department of Internal Medicine, University of California Medical Center, Sacramento, CA 95817, USA

[2]Department of Molecular and Cell Biology, 251 LSA, University of California, Berkeley, CA 94720-3200, USA

关键词: Skin;    Ultraviolet light;    Ozone;    Oxidative stress;    Carbonyl;   

DOI  :  10.1016/S0014-5793(98)00049-0

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The presence of stratum corneum carbonyls may serve as an intrinsic dosimeter for environmental oxidative damage to skin. To investigate the accumulation of carbonyls in human stratum corneum, skin was tape-stripped, then tapes were sequentially incubated with 2,4-dinitrophenyl hydrazine (DNPH), rat anti-DNP, mouse anti-rat IgG conjugated with alkaline phosphatase, p-nitrophenyl phosphate and absorbance (405 nm) measured and carbonyls estimated. Stratum corneum exposed in vitro to oxidants: hypochlorous acid (1, 10, 100 mM), ozone (0, 1, 5, 10 ppm for 2 h) or UV light (280–400 nm; 0, 4, 88, or 24 J/cm²) contained increased carbonyls. Furthermore, stratum corneum carbonyls were elevated in tanned compared with untanned sites: dorsal hand (0.43±0.06 nmol/cm²) vs. lower arm (0.32±0.04, mean±S.E.M., n=11; P<0.003) and lower back (0.26±0.02) vs. buttock (0.21±0.02; n=6, P<0.01) indicating in vivo oxidative damage.

【 授权许可】

Unknown