Bystander effectors of chondrosarcoma cells irradiated at different LET impair proliferation of chondrocytes

Journal of Cell Communication and Signaling

article

Lepleux, Charlotte¹  Saintigny, Yannick¹  Savu, Diana³  Chevalier, François¹  Marie-Brasset, Aurélie¹  Temelie, Mihaela¹  Boulanger, Marion¹  Brotin, Émilie⁴  Goldring, Mary B.⁵  Hirtz, Christophe⁶  Varès, Guillaume⁷  Nakajima, Tetsuo⁸ 

[1] François Jacob Institute of biology;CEA - CNRS - ENSICAEN - Université de Caen Normandie;Department of Life and Environmental Physics, Horia Hulubei National Institute of Physics and Nuclear Engineering;Inserm U1086 ANTICIPE « Interdisciplinary Research Unit for Cancer Prevention and Treatment », Comprehensive Cancer Center F. Baclesse;Research Division, Hospital for Special Surgery and Weill Cornell Medical College;University of Montpellier;Cell Signal Unit;Dept. of Radiation Effects Research

关键词: Chondrosarcoma;    Radiation-induced bystander effect;    Chondrocyte;    High-LET;    Low-dose irradiation;   

DOI  :  10.1007/s12079-019-00515-9

学科分类：分子生物学，细胞生物学和基因

来源: Springer

【 摘 要 】

While the dose-response relationship of radiation-induced bystander effect (RIBE) is controversial at low and high linear energy transfer (LET), mechanisms and effectors of cell-to-cell communication stay unclear and highly dependent of cell type. In the present study, we investigated the capacity of chondrocytes in responding to bystander factors released by chondrosarcoma cells irradiated at different doses (0.05 to 8 Gy) with X-rays and C-ions. Following a medium transfer protocol, cell survival, proliferation and DNA damages were quantified in bystander chondrocytes. The bystander factors secreted by chondrosarcoma cells were characterized. A significant and major RIBE response was observed in chondrocyte cells (T/C-28a2) receiving conditioned medium from chondrosarcoma cells (SW1353) irradiated with 0.1 Gy of X-rays and 0.05 Gy of C-ions, resulting in cell survivals of 36% and 62%, respectively. Micronuclei induction in bystander cells was observed from the same low doses. The cell survival results obtained by clonogenic assays were confirmed using impedancemetry. The bystander activity was vanished after a heat treatment or a dilution of the conditioned media. The cytokines which are well known as bystander factors, TNF-α and IL-6, were increased as a function of doses and LET according to an ELISA multiplex analysis. Together, the results demonstrate that irradiated chondrosarcoma cells can communicate stress factors to non-irradiated chondrocytes, inducing a wide and specific bystander response related to both doses and LET.

【 授权许可】

CC BY