Protective Effect of CpG-Oligodeoxynucleotides Against Low- and High-LET Irradiation

Cellular Physiology and Biochemistry

关键词: CpG-ODNs;    Irradiation;    Carbon beam;    High-LET;   

DOI  :  10.1159/000366368

学科分类：分子生物学，细胞生物学和基因

来源: S Karger AG

【 摘 要 】

Background/Aims CpG-oligodeoxynucleotides (ODNs) are synthetic DNA sequences containing unmethylated cytosine-guanine motifs with potent immunomodulatory effects. Previous reports showed a powerful protective effect of CpG-ODN against the damage induced by low-LET γ-rays. In this study, we explored whether CpG-ODN also protects against the damage induced by high-LET irradiation. Parallel experiments were performed with low-LET irradiation. Methods RAW264.7 cells were incubated with 1 μM of CpG-ODN after γ-ray or carbon-beam irradiation. Cell death was then measured by PI/DAPI double staining, cell survival was assessed by colony-formation assays, DNA damage was evaluated by comet assays, cell cycle was monitored by flow cytometry, and the levels of apoptosis-related proteins were detected by western blots. Results When irradiated cells were treated with the CpG-ODN, cell viability decreased, cell survival increased, DNA damage and G₂/M-phase arrest were ameliorated, and apoptosis was inhibited. Conclusions The CpG-ODN showed protective effects against low-LET γ-ray and high-LET carbon-beam irradiation. These effects might be associated with the repair of DNA damage and inhibition of apoptosis.

【 授权许可】

CC BY-NC-ND