Nasopharyngeal Microbiota in SARS-CoV-2 Positive and Negative Patients

Biological Procedures Online

Alan Landay¹  Cheryl Jennings²  Stefan J. Green³  Ankur Naqib⁴  Phillip A. Engen⁴  Robin M. Voigt⁵  Ali Keshavarzian⁶ 

[1] Department of Internal Medicine, Division of Geriatrics and Palliative Medicine, Rush Medical College, Chicago, IL, USA;Department of Molecular Pathogens and Immunity, Rush University Medical Center, Chicago, IL, USA;Genomics and Microbiome Core Facility, Rush University Medical Center, Chicago, IL, USA;Rush Medical College, Rush Center for Integrated Microbiome and Chronobiology Research, Rush University Medical Center, 1725 W. Harrison St. STE 206, 60612, Chicago, IL, USA;Rush Medical College, Rush Center for Integrated Microbiome and Chronobiology Research, Rush University Medical Center, 1725 W. Harrison St. STE 206, 60612, Chicago, IL, USA;Department of Medicine, Rush University Medical Center, Chicago, IL, USA;Rush Medical College, Rush Center for Integrated Microbiome and Chronobiology Research, Rush University Medical Center, 1725 W. Harrison St. STE 206, 60612, Chicago, IL, USA;Department of Physiology, Rush University Medical Center, Chicago, IL, USA;Department of Medicine, Rush University Medical Center, Chicago, IL, USA;

关键词: SARS-CoV-2;    COVID-19;    Nasopharyngeal;    Microbiota;    Dysbiosis;   

DOI  :  10.1186/s12575-021-00148-6

来源: Springer

【 摘 要 】

We investigated nasopharyngeal microbial community structure in COVID-19-positive and -negative patients. High-throughput 16S ribosomal RNA gene amplicon sequencing revealed significant microbial community structure differences between COVID-19-positive and -negative patients. This proof-of-concept study demonstrates that: (1) nasopharyngeal microbiome communities can be assessed using collection samples already collected for SARS-CoV-2 testing (viral transport media) and (2) SARS-CoV-2 infection is associated with altered dysbiotic microbial profiles which could be a biomarker for disease progression and prognosis in SARS-CoV-2.

【 授权许可】

CC BY