An Overview of Nucleic Acid Testing for the Novel Coronavirus SARS-CoV-2

Frontiers in Medicine

Huiling Wang¹  Gang Li¹  Yanjie Li¹  Jing Zhao¹  Yushu Ai¹ 

[1] Department of Clinical Laboratory, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, China;Department of Research Management, Henan Provincial People's Hospital, People's Hospital of Zhengzhou University, People's Hospital of Henan University, Zhengzhou, China;

关键词: COVID-19;    nucleic acid testing;    SARS-CoV-2;    qRT-PCR;    specimen;   

DOI  :  10.3389/fmed.2020.571709

来源: Frontiers

【 摘 要 】

In this note we analyze the problems in the nucleic acid testing (NAT) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), and we also give some suggestions for improving the accuracy of NAT diagnosis. NAT testing is considered to be the diagnostic “gold standard”; at present there are few reviews on NAT for SARS-CoV-2. Moreover, many false-negative results always appear in the procedure of detecting, which has affected early diagnosis of the disease and brought a great challenge to mitigation and containment of the pandemic. In conclusion, comprehensive analyses of serological and imaging findings should be performed to guide the formulation of an accurate clinical diagnosis, treatment plan, and monitoring therapeutic efficacy, in an effort to achieve early diagnosis, containment, and treatment of the disease, thereby effectively reducing progression of the pandemic. This article presents a literature overview of SARS-CoV-2 nucleic acid testing, aiming to provide support for clinicians.

【 授权许可】

CC BY