Promises and challenges of nanoplasmonic devices for refractometric biosensing

Nanophotonics

article

Andreas B. Dahlin¹  Nathan J. Wittenberg³  Fredrik Höök¹  Sang-Hyun Oh³ 

[1] Division of Bionanophotonics, Department of Applied Physics, Chalmers University of Technology;These authors contributed equally to this work.;Laboratory of Nanostructures and Biosensing, Department of Electrical and Computer Engineering, University of Minnesota;Department of Biophysics and Chemical Biology, Seoul National University

关键词: Optical biosensors;    refractometric sensors;    surface plasmon resonance;    plasmonics;    figure of merit;    single molecule detection;    enzyme-linked biosensing;    site-specific chemistry;    supported lipid bilayer;    pore-spanning lipid membrane;    nanoparticle;    nanohole;    optofluidics;   

DOI  :  10.1515/nanoph-2012-0026

学科分类：社会科学、人文和艺术（综合）

来源: De Gruyter

【 摘 要 】

Optical biosensors based on surface plasmon resonance (SPR) in metallic thin films are currently standard tools for measuring molecular binding kinetics and affinities – an important task for biophysical studies and pharmaceutical development. Motivated by recent progress in the design and fabrication of metallic nanostructures, such as nanoparticles or nanoholes of various shapes, researchers have been pursuing a new generation of biosensors harnessing tailored plasmonic effects in these engineered nanostructures. Nanoplasmonic devices, while demanding nanofabrication, offer tunability with respect to sensor dimension and physical properties, thereby enabling novel biological interfacing opportunities and extreme miniaturization. Here we provide an integrated overview of refractometric biosensing with nanoplasmonic devices and highlight some recent examples of nanoplasmonic sensors capable of unique functions that are difficult to accomplish with conventional SPR. For example, since the local field strength and spatial distribution can be readily tuned by varying the shape and arrangement of nanostructures, biomolecular interactions can be controlled to occur in regions of high field strength. This may improve signal-to-noise and also enable sensing a small number of molecules. Furthermore, the nanoscale plasmonic sensor elements may, in combination with nanofabrication and materials-selective surface-modifications, make it possible to merge affinity biosensing with nanofluidic liquid handling.

【 授权许可】

CC BY