Biocomposite scaffold preparation from hydroxyapatite extracted from waste bovine bone

Green Processing and Synthesis

article

Quoc-Phong Ho¹  The-Duong Tao¹  Lien-Huong Huynh¹  Meng-Jiy Wang² 

[1] Department of Chemical Engineering, College of Engineering Technology, Can Tho University;Department of Chemical Engineering, National Taiwan University of Science and Technology

关键词: bovine bone;    hydroxyapatite;    phase separation scaffold;    tissue engineering;   

DOI  :  10.1515/gps-2020-0005

来源: De Gruyter

【 摘 要 】

This study was conducted to fabricate scaffold from polylactic acid (PLA) and hydroxyapatite (HA) extracted from waste bovine bone for enhancing both mechanical and biocompatible properties. After pretreatment in dilute NaOH solution, the bone was calcined at 900°C for 6 h, ball milled and converted to HA. Factors that affect the formation of HA were investigated. Experimental results showed that HA particles with crystal size < 100 nm and 99% crystallinity could be obtained at 90°C, pH 11 and 35 mM H 3 PO 4 solution followed by 4 h calcination at 900°C. By using non-solvent induced phase separation method, PLA scaffolds with pore size and surface area of 22.6 μm and 25.7 m 2 /g, respectively, containing different hydroxyapatite were successfully prepared. Tensile strength of scaffolds increased due to effective support by HA grafted collagen. PLA scaffolds containing HA were more degradable than PLA scaffolds and PLA scaffolds containing HA grafted collagen. Cell culture results showed that cell density increased significantly on porous scaffolds than that on the dense scaffolds. Moreover, cell density also increased significantly on the scaffold containing HA grafted collagen than that on the scaffold with pure HA.

【 授权许可】

CC BY