Morphological and chemical analysis of bone substitutes by scanning electron microscopy and microanalysis by spectroscopy of dispersion energy

Brazilian Dental Journal

Gabriela Alessandra Da Cruz¹  Sérgio De Toledo¹  Enilson Antonio Sallum¹  Antonio Fernando Martorelli De Lima¹ 

[1] ,State University of Campinas School of Dentistry of Piracicaba Department of Prosthodontics and PeriodonticsPiracicaba SP ,Brazil

关键词: scanning electron microscopy;    x-ray microanalysis;    bone substitute;    bovine bone;    human bone;    hydroxyapatite;   

DOI  :  10.1590/S0103-64402007000200008

来源: SciELO

【 摘 要 】

This study evaluated the morphological and chemical composition of the following bone substitutes: cancellous and cortical organic bovine bone with macro and microparticle size ranging from 1.0 to 2.0 mm and 0.25 to 1.0 mm, respectively; inorganic bovine bone with particle size ranging from 0.25 to 1.0 mm; hydroxyapatite with particle size ranging from 0.75 to 1.0 mm; and demineralized freeze-dried bone allograft with particle size ranging from 0.25 to 0.5 mm. The samples were sputter-coated with gold in an ion coater, the morphology was observed and particle size was measured under vacuum by scanning electron microscopy (SEM). The chemical composition was evaluated by spectroscopy of dispersion energy (EDS) microanalysis using samples without coating. SEM analysis provided visual evidence that all examined materials have irregular shape and particle sizes larger than those informed by the manufacturer. EDS microanalysis detected the presence of sodium, calcium and phosphorus that are usual elements of the bone tissue. However, mineral elements were detected in all analyzed particles of organic bovine bone except for macro cancellous organic bovine bone. These results suggest that the examined organic bovine bone cannot be considered as a pure organic material.

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