Activation of Human Osteoblasts via Different Bovine Bone Substitute Materials With and Without Injectable Platelet Rich Fibrin in vitro

Frontiers in Bioengineering and Biotechnology

Diana Heimes¹  Daniel G. E. Thiem¹  Sebastian Blatt¹  Keyvan Sagheb¹  Bilal Al-Nawas¹  Solomiya Kyyak¹  Eik Schiegnitz¹  Peer W. Kämmerer¹  Henning Staedt² 

[1] Department of Oral- and Maxillofacial Surgery, University Medical Center Mainz, Mainz, Germany;Department of Prosthodontics and Materials Science, University Medical Center Rostock, Rostock, Germany;Private Practice, University Medical Center Rostock, Rostock, Germany;

关键词: bone substitute;    bovine bone;    platelet rich fibrin (PRF);    in vitro;    osteoblast;    vitality;   

DOI  :  10.3389/fbioe.2021.599224

来源: DOAJ

【 摘 要 】

IntroductionThe aim of the in vitro study was to compare the effect of four bovine bone substitute materials (XBSM) with and without injectable platelet-reach fibrin for viability and metabolic activity of human osteoblasts (HOB) as well as expression of alkaline phosphatase (ALP), bone morphogenetic protein 2 (BMP-2), and osteonectin (OCN).Materials and MethodsCerabone® (CB), Bio-Oss® (BO), Creos Xenogain® (CX) and MinerOss® X (MO) ± i-PRF were incubated with HOB. At day 3, 7, and 10, cell viability and metabolic activity as well as expression of ALP, OCN, and BMP-2, was examined.ResultsFor non-i-PRF groups, the highest values concerning viability were seen for CB at all time points. Pre-treatment with i-PRF increased viability in all groups with the highest values for CB-i-PRF after 3 and 7 and for CX-i-PRF after 10 days. For metabolic activity, the highest rate among non-i-PRF groups was seen for MO at day 3 and for CB at day 7 and 10. Here, i-PRF groups showed higher values than non-i-PRF groups (highest values: CB + i-PRF) at all time points. There was no difference in ALP-expression between groups. For OCN expression in non-i-PRF groups, CB showed the highest values after day 3, CX after day 7 and 10. Among i-PRF-groups, the highest values were seen for CX + i-PRF. At day 3, the highest BMP-2 expression was observed for CX. Here, for i-PRF groups, the highest increase was seen for CX + i-PRF at day 3. At day 7 and 10, there was no significant difference among groups.ConclusionXBSM sintered under high temperature showed increased HOB viability and metabolic activity through the whole period when compared to XBSM manufactured at lower temperatures. Overall, the combination of XBSM with i-PRF improved all cellular parameters, ALP and BMP-2 expression at earlier stages as well as OCN expression at later stages.

【 授权许可】

Unknown