Protective effect of hydrogen‐rich saline against radiation‐induced immune dysfunction

Journal of Cellular and Molecular Medicine

Sanhu Zhao¹  Yanyong Yang²  Wen Liu²  Zhiqiang Xuan¹  Shouming Wu¹  Shunfei Yu¹  Ke Mei³  Yijuan Huang²  Pei Zhang²  Jianming Cai²  Jin Ni² 

[1] Zhejiang Provincial Center for Disease Control and Prevention, Zhejiang, China;Department of Radiation Medicine, Second Military Medical University, Shanghai, China;Department of Radiology, Shanghai First People's Hospital, Shanghai, China

关键词: ionizing radiation;    hydrogen;    radioprotection;    apoptosis;    immune regulation;   

DOI  :  10.1111/jcmm.12245

来源: Wiley

【 摘 要 】

Abstract

Recent studies showed that hydrogen can be used as an effective radioprotective agent through scavenging free radicals. This study was undertaken to evaluate the radioprotective effects of hydrogen on immune system in mice. H₂ was dissolved in physiological saline using an apparatus produced by our department. Spleen index and histological analysis were used to evaluate the splenic structural damage. Spleen superoxide dismutase, GSH, MDA were measured to appraise the antioxidant capacity and a DCF assay for the measurement of radical oxygen species. Cell apoptosis was evaluated by an Annexin V-FITC and propidium iodide staining method as well as the apoptotic proteins such as Bcl-2, Bax, caspase-3 and c-caspase-3. CD4+ and CD8+ T cells subtypes were detected by flow cytometry with FITC-labelled antimouse CD4 and PE antimouse CD8 staining. Real-time PCR was utilized to determine the CD4+ T cell subtypes and related cytokines. Our study demonstrated that pre-treatment with H₂ could increase the spleen index and attenuate the radiation damage on splenic structure. Radical oxygen species level was also reduced by H₂ treatment. H₂ also inhibited radiation-induced apoptosis in splenocytes and down-regulated pro-apoptotic proteins in living mice. Radiation-induced imbalance of T cells was attenuated by H₂. Finally, we found that H₂ could regulate the polarization of CD4+ T cells and the level of related cytokines. This study suggests H₂ as an effective radioprotective agent on immune system by scavenging reactive oxygen species.

【 授权许可】

CC BY   
© 2014 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.