Mapping and determinism of soil microbial community distribution across an agricultural landscape

MicrobiologyOpen

Florentin Constancias¹  Sébastien Terrat⁵  Nicolas P. A. Saby²  Walid Horrigue⁵  Jean Villerd³  Jean-Philippe Guillemin⁴  Luc Biju-Duval¹  Virginie Nowak¹  Samuel Dequiedt⁵  Lionel Ranjard¹ 

[1] INRA, UMR1347 Agroécologie, Dijon, France;INRA, US1106 InfoSol, Orleans, France;INRA, UMR1121 Universite de Lorraine (Ensaia), Vandoeuvre-les-Nancy, France;AgroSup Dijon, UMR1347 Agroecologie, Dijon, France;INRA, UMR1347 Agroécologie-Plateforme GenoSol, Dijon, France

关键词: Agricultural practices;    bacterial diversity;    environmental filters;    landscape;    mapping;    soil microbial ecology;   

DOI  :  10.1002/mbo3.255

来源: Wiley

【 摘 要 】

Abstract

Despite the relevance of landscape, regarding the spatial patterning of microbial communities and the relative influence of environmental parameters versus human activities, few investigations have been conducted at this scale. Here, we used a systematic grid to characterize the distribution of soil microbial communities at 278 sites across a monitored agricultural landscape of 13 km². Molecular microbial biomass was estimated by soil DNA recovery and bacterial diversity by 16S rRNA gene pyrosequencing. Geostatistics provided the first maps of microbial community at this scale and revealed a heterogeneous but spatially structured distribution of microbial biomass and diversity with patches of several hundreds of meters. Variance partitioning revealed that both microbial abundance and bacterial diversity distribution were highly dependent of soil properties and land use (total variance explained ranged between 55% and 78%). Microbial biomass and bacterial richness distributions were mainly explained by soil pH and texture whereas bacterial evenness distribution was mainly related to land management. Bacterial diversity (richness, evenness, and Shannon index) was positively influenced by cropping intensity and especially by soil tillage, resulting in spots of low microbial diversity in soils under forest management. Spatial descriptors also explained a small but significant portion of the microbial distribution suggesting that landscape configuration also shapes microbial biomass and bacterial diversity.

【 授权许可】

CC BY   
© 2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

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