Pyrosequencing analysis of a bacterial community associated with lava‐formed soil from the Gotjawal forest in Jeju, Korea

MicrobiologyOpen

Jong-Shik Kim²  Keun Chul Lee¹  Dae-Shin Kim³  Suk-Hyung Ko³  Man-Young Jung⁴  Sung-Keun Rhee⁴ 

[1] Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea;Gyeongbuk Institute for Marine Bioindustry, Uljin, Korea;Research Institute for Hallasan, Jeju Special Self-Governing Province, Korea;Department of Microbiology, Chungbuk National University, Cheongju, Korea

关键词: 16S rRNA gene;    bacterial diversity;    Gotjawal forest soil;    pyrosequencing;   

DOI  :  10.1002/mbo3.238

来源: Wiley

【 摘 要 】

Abstract

In this study, we analyzed the bacterial diversity in soils collected from Gyorae Gotjawal forest, where globally unique topography, geology, and ecological features support a forest grown on basalt flows from 110,000 to 120,000 years ago and 40,000 to 50,000 years ago. The soils at the site are fertile, with rocky areas, and are home to endangered species of plants and animals. Rainwater penetrates to the groundwater aquifer, which is composed of 34% organic matter containing rare types of soil and no soil profile. We determined the bacterial community composition using 116,475 reads from a 454-pyrosequencing analysis. This dataset included 12,621 operational taxonomic units at 3% dissimilarity, distributed among the following groups: Proteobacteria (56.2%) with 45.7% of α-Proteobacteria, Actinobacteria (25%), Acidobacteria (10.9%), Chloroflexi (2.4%), and Bacteroidetes (0.9%). In addition, 16S rRNA gene sequences were amplified using polymerase chain reaction and domain-specific primers to construct a clone library based on 142 bacterial clones. These clones were affiliated with the following groups: Proteobacteria (56%) with 51% of α-Proteobacteria, Acidobacteria (7.8%), Actinobacteria (17.6%), Chloroflexi (2.1%), Bacilli (1.4%), Cyanobacteria (2.8%), and Planctomycetes (1.4%). Within the phylum Proteobacteria, 56 of 80 clones were tentatively identified as 12 unclassified genera. Several new genera and a new family were discovered within the Actinobacteria clones. Results from 454-pyrosequencing revealed that 57% and 34% of the sequences belonged to undescribed genera and families, respectively. The characteristics of Gotjawal soil, which are determined by lava morphology, vegetation, and groundwater penetration, might be reflected in the bacterial community composition.

【 授权许可】

CC BY   
© 2015 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.