Internal comparison between deuterium oxide (D2O) and L‐[ring‐13C6] phenylalanine for acute measurement of muscle protein synthesis in humans

Physiological Reports

Daniel J. Wilkinson¹  Jessica Cegielski¹  Bethan E. Phillips¹  Catherine Boereboom¹  Jonathan N. Lund¹  Philip J. Atherton¹ 

[1] MRC-ARUK Centre of Excellence for Musculoskeletal Ageing Research, School of Medicine, University of Nottingham, Derby, UK

关键词: Deuterium oxide;    metabolism;    muscle protein synthesis;    stable isotope tracers;   

DOI  :  10.14814/phy2.12433

来源: Wiley

【 摘 要 】

Abstract

Stable isotope tracer methodologies are becoming increasingly widespread in metabolic research; yet a number of factors restrict their implementation, such as, i.v infusions, multiple cannulae, tissue samples, and significant cost. We recently validated the sensitivity of the orally administered stable isotope tracer deuterium oxide (D₂O) for quantifying day-to-day changes in muscle protein synthesis (MPS). This method is less invasive, restrictive, and more cost-effective than traditional amino acid (AA) tracer techniques. In the present study, we hypothesized the sensitivity of our analytical techniques (GC-Pyrolysis-IRMS) would permit D₂O-derived measurements of MPS over much shorter periods (i.e., hours) usually only possible using AA-tracer techniques. We recruited nine males (24 ± 3 year, BMI: 25 ± 3 kg·m⁻²) into an internally controlled comparison of D₂O versus ¹³C AA-tracers. The day before the acute study subjects consumed 400 mL D₂O, and on the study day, received a primed (0.3 mg·kg⁻¹) continuous (0.6 mg·kg·h⁻¹) i.v infusion of L-[ring-¹³C₆]-phenylalanine to quantify MPS under both: (1) basal [postabsorptive] and; (2) stimulated [postprandial] that is, consumption of 20 g EAA, conditions. Measures of MPS yielded indistinguishable technique differences with respect to EAA, ¹³C: 0.065 ± 0.004 to 0.089 ± 0.006%·h⁻¹ (P < 0.05) and D₂O: 0.050 ± 0.007 to 0.088 ± 0.008%·h⁻¹ (P < 0.05) with qualitatively similar increases. Our findings reveal that acute measurement of MPS, usually only possible using AA-tracers, are feasible over shorter periods with orally administered D₂O when used in tandem with GC-Pyrolysis-IRMS. We conclude that this D₂O approach provides a less invasive, cost-effective, and flexible means by which to quantify MPS acutely over several hours.

【 授权许可】

CC BY   
© 2015 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of the American Physiological Society and The Physiological Society.

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