Differential roles of astrocyte and microglia in supporting oligodendrocyte development and myelination in vitro

Brain and Behavior

Yi Pang⁵  Lir-Wan Fan⁵  Lu-Tai Tien¹  XueMei Dai⁴  Baoying Zheng²  Zhengwei Cai⁵  Rick C. S. Lin³ 

[1] School of Medicine, Fu Jen Catholic University, New Taipei City, Taiwan;Department of Pathology, University of Mississippi Medical Center, Jackson, Mississippi;Department of Neurobiology and Anatomical Sciences, University of Mississippi Medical Center, Jackson, Mississippi;Department of Chemistry, Jackson State University, Jackson, Mississippi, 39217;Department of Pediatrics, University of Mississippi Medical Center, Jackson, Mississippi

关键词: Cytokine;    differentiation;    glia;    in vitro;    proliferation;   

DOI  :  10.1002/brb3.152

来源: Wiley

【 摘 要 】

Abstract

Oligodendrocyte (OL) development relies on many extracellular cues, most of which are secreted cytokines from neighboring neural cells. Although it is generally accepted that both astrocytes and microglia are beneficial for OL development, there is a lack of understanding regarding whether astrocytes and microglia play similar or distinct roles. The current study examined the effects of astrocytes and microglia on OL developmental phenotypes including cell survival, proliferation, differentiation, and myelination in vitro. Our data reveal that, although both astrocytes- and microglia-conditioned medium (ACDM and MCDM, respectively) protect OL progenitor cells (OPCs) against growth factor withdrawal-induced apoptosis, ACDM is significantly more effective than MCDM in supporting long-term OL survival. In contrast, MCDM preferentially promotes OL differentiation and myelination. These differential effects of ACDM and MCDM on OL development are highlighted by distinct pattern of cytokine/growth factors in the conditioned medium, which correlates with differentially activated intracellular signaling pathways in OPCs upon exposure to the conditioned medium.

【 授权许可】

CC BY   
© 2013 The Authors. Brain and Behavior published by Wiley Periodicals, Inc.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.