Neurogenin3 Participates in Gliogenesis in the Developing Vertebrate Spinal Cord

Developmental Biology

Ying Liu¹  David Scheel¹  Yingchuan Qi¹  Haipeng Xue¹  Francois Guillemot¹  Yuanyuan Wu¹  Michael German¹  Mengsheng Qiu¹  Jeffrey Lee¹  Mahendra Rao¹ 

[1] Department of Neurobiology and Anatomy, University of Utah School of Medicine, 50 North Medical Drive, Salt Lake City, Utah, 84132

关键词: bHLH;    homeodomain;    Ngn3;    Nkx2.2;    development;    differentiation;    oligodendrocytes;    astrocytes;    glia;    spinal cord;   

DOI  :  10.1006/dbio.2002.0868

学科分类：生物科学（综合）

来源: Academic Press

【 摘 要 】

Tostudytheroleofbasichelix–loop–helix(bHLH)transcriptionfactorsingliogenesis,weexaminedwhetherbHLHtranscriptionfactorswereexpressedinglialprecursorcellsandparticipatedinregulatingoligodendrocyteandastrocytedevelopment.Asassessedbyreversetranscription–polymerasechainreaction(RT-PCR),Neurogenin3(Ngn3)wastransientlyexpressedinbipotentialglialcellsfatedtobecomeeitheroligodendrocytesorastrocytes.MicelackingNgn3displayedalossofNkx2.2expression,atranscriptionfactorrequiredforproperoligodendrogliogenesis.Furthermore,areductionintheexpressionofmyelinbasicprotein(MBP),proteolipidprotein(PLP),andglialfibrillaryacidicprotein(GFAP),markersformatureoligodendrocytesandastrocytes,wasobservedintheNgn3nullmice.OverexpressionofNgn3wassufficienttodriveexpressionfromthePLPpromoterintransientcotransfectionassays.Overall,thedatasuggestthatNgn3mayregulateglialdifferentiationatadevelopmentalstagepriortothesegregationoftheoligodendrocyteandastrocytelineage.

【 授权许可】

Unknown