Hyaluronic acid-based bioink improves the differentiation and network formation of neural progenitor cells

Frontiers in Bioengineering and Biotechnology

Bioengineering and Biotechnology

Josep M. Canals¹  Clelia Introna¹  Inês Pereira²  Aranzazu Villasante³  Josep Samitier⁴  Maria J. Lopez-Martinez⁴  Daniel Tornero⁵ 

[1] Laboratory of Stem Cells and Regenerative Medicine, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Institute of Neurosciences, University of Barcelona, Barcelona, Spain;Creatio - Production and Validation Center of Advanced Therapies, Faculty of Medicine and Health Sciences, University of Barcelona, Barcelona, Spain;Research Foundation Clinic Barcelona-August Pi i Sunyer Biomedical Research Institute (FRCB-IDIBAPS), Barcelona, Spain;Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain;Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain;Department of Electronic and Biomedical Engineering, University of Barcelona, Barcelona, Spain;Nanobioengineering Group, Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology (BIST), Barcelona, Spain;Department of Electronic and Biomedical Engineering, University of Barcelona, Barcelona, Spain;Biomedical Research Networking, Center in Bioengineering, Biomaterials and Nanomedicine (CIBER-BBN), Madrid, Spain;Research Foundation Clinic Barcelona-August Pi i Sunyer Biomedical Research Institute (FRCB-IDIBAPS), Barcelona, Spain;Laboratory of Neuronal Stem Cells and Cerebral Damage, Department of Biomedical Sciences, Faculty of Medicine and Health Sciences, Institute of Neurosciences, University of Barcelona, Barcelona, Spain;

关键词: bioprinting;    biomaterials;    in vitro;    neural progenitor cells;    differentiation;    neuronal models;   

DOI  :  10.3389/fbioe.2023.1110547

 received in 2022-11-29, accepted in 2023-02-15,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: Three-dimensional (3D) bioprinting is a promising technique for the development of neuronal in vitro models because it controls the deposition of materials and cells. Finding a biomaterial that supports neural differentiation in vitro while ensuring compatibility with the technique of 3D bioprinting of a self-standing construct is a challenge.Methods: In this study, gelatin methacryloyl (GelMA), methacrylated alginate (AlgMA), and hyaluronic acid (HA) were examined by exploiting their biocompatibility and tunable mechanical properties to resemble the extracellular matrix (ECM) and to create a suitable material for printing neural progenitor cells (NPCs), supporting their long-term differentiation. NPCs were printed and differentiated for up to 15 days, and cell viability and neuronal differentiation markers were assessed throughout the culture.Results and Discussion: This composite biomaterial presented the desired physical properties to mimic the ECM of the brain with high water intake, low stiffness, and slow degradation while allowing the printing of defined structures. The viability rates were maintained at approximately 80% at all time points. However, the levels of β-III tubulin marker increased over time, demonstrating the compatibility of this biomaterial with neuronal cell culture and differentiation. Furthermore, these cells showed increased maturation with corresponding functional properties, which was also demonstrated by the formation of a neuronal network that was observed by recording spontaneous activity via Ca2+ imaging.

【 授权许可】

Unknown   
Copyright © 2023 Pereira, Lopez-Martinez, Villasante, Introna, Tornero, Canals and Samitier.