Methionine restriction slows down senescence in human diploid fibroblasts

Aging Cell

Rafał Kozieł¹  Christoph Ruckenstuhl²  Eva Albertini¹  Michael Neuhaus¹  Christine Netzberger²  Maria Bust³  Frank Madeo²  Rudolf J. Wiesner³ 

[1] Institute for Biomedical Aging Research (IBA), Universität Innsbruck, Innsbruck, Austria;Institute of Molecular Biosciences, Karl-Franzens University, Graz, Austria;Institute for Vegetative Physiology, University of Köln, Köln, Germany

关键词: cellular senescence;    fibroblast;    methionine;    mitochondria;    oxidative stress;   

DOI  :  10.1111/acel.12266

来源: Wiley

【 摘 要 】

Summary

Methionine restriction (MetR) extends lifespan in animal models including rodents. Using human diploid fibroblasts (HDF), we report here that MetR significantly extends their replicative lifespan, thereby postponing cellular senescence. MetR significantly decreased activity of mitochondrial complex IV and diminished the accumulation of reactive oxygen species. Lifespan extension was accompanied by a significant decrease in the levels of subunits of mitochondrial complex IV, but also complex I, which was due to a decreased translation rate of several mtDNA-encoded subunits. Together, these findings indicate that MetR slows down aging in human cells by modulating mitochondrial protein synthesis and respiratory chain assembly.

【 授权许可】

CC BY   
© 2014 The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.

Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.