Monitoring of cellular senescence by DNA‐methylation at specific CpG sites

Aging Cell

Carmen M. Koch¹  Sylvia Joussen¹  Anne Schellenberg¹  Qiong Lin¹  Martin Zenke¹ 

[1] Helmholtz-Institute for Biomedical Engineering, Stem Cell Biology and Cellular Engineering

关键词: cellular senescence;    epigenetic;    DNA‐methylation;    mesenchymal stem cells;    fibroblast;   

DOI  :  10.1111/j.1474-9726.2011.00784.x

来源: Wiley

【 摘 要 】

Summary

Replicative senescence has fundamental implications on cell morphology, proliferation, and differentiation potential. Here, we describe a simple method to track long-term culture based on continuous DNA-methylation changes at six specific CpG sites. This epigenetic senescence signature can be used as biomarker for various cell types to predict the state of cellular senescence with regard to the number of passages, population doublings, or days of in vitro culture.

【 授权许可】

Unknown   
© 2011 The Authors. Aging Cell © 2011 Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland