Case Report: Molecular Diagnosis of Fungal Keratitis Associated With Contact Lenses Caused by Fusarium solani

Frontiers in Medicine

Laura Trovato¹  Salvatore Oliveri¹  Antonio Marino²  Giovanni Pizzo² 

[1] Laboratory Analysis Unit, Azienda Ospedaliero Universitaria Policlinico “G. Rodolico-San Marco”, Catania, Italy;Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy;Ophthalmology Unit, Azienda di Rilievo Nazionale e di Alta Specializzazione Garibaldi, Catania, Italy;

关键词: Fungal keratitis;    Fusarium solani;    contact lenses;    diagnosis;    PCR;   

DOI  :  10.3389/fmed.2021.579516

来源: Frontiers

【 摘 要 】

Fusarium is a filamentous fungus commonly found in the environment and is the major cause of fungal keratitis. We report a case of keratomycosis caused by Fusarium solani in a patient using disposable soft contact lenses. A delay in diagnosis led to the initiation of an empirical antifungal treatment with the subsequent deterioration of the patient's clinical condition. The use of the real-time quantitative PCR assay confirmed keratitis from F. solani providing a result in <48 h and therefore giving the possibility of quickly starting targeted antifungal therapy. The patient had an improvement in eye condition after the diagnosis of keratitis by F. solani and the rapid change to targeted antifungal treatment. For the rapid identification of corneal fungal pathogens, we believe that PCR may be added for the diagnosis of mycotic keratitis pending the isolation in culture that is necessary for in vitro susceptibility testing.

【 授权许可】

CC BY