A Comprehensive Review of Fluorescence Correlation Spectroscopy

Frontiers in Physics

Juanjuan Zheng¹  Lan Yu¹  Min Liu¹  Peng Gao¹  Yunze Lei¹  Ying Ma¹  Dan Dan¹ 

[1] Xi'an, China;

关键词: fluorescence correlation spectroscopy;    biomolecular dynamics;    biomolecular interaction;    correlation spectroscopy;    FCS variants;   

DOI  :  10.3389/fphy.2021.644450

来源: Frontiers

【 摘 要 】

Fluorescence correlation spectroscopy (FCS) is a powerful technique for quantification of molecular dynamics, and it has been widely applied in diverse fields, e.g., biomedicine, biophysics, and chemistry. By time-correlation of the fluorescence fluctuations induced by molecules diffusing through a focused light, FCS can quantitatively evaluate the concentration, diffusion coefficient, and interaction of the molecules in vitro or in vivo. In this review, the basic principle and implementation of FCS are introduced. Then, the advances of FCS variants are reviewed, covering dual-color FCCS, multi-focus FCS, pair correlation function (pCF), scanning FCS, focus-reduced FCS, SPIM-FCS, and inverse-FCS. Besides, the applications of FCS are demonstrated with the measurement of local concentration, hydrodynamic radius, diffusion coefficient, and the interaction of different molecules. Lastly, a discussion is given by summarizing the pros and cons of different FCS techniques, as well as the outlooks and perspectives of FCS.

【 授权许可】

CC BY