NMR structure of the haem core of a novel tetrahaem cytochrome isolated from Shewanella frigidimarina: identification of the haem‐specific axial ligands and order of oxidation

FEBS Letters

Cuthbertson, Pauline M²  Pessanha, Miguel³  Reid, Graeme A²  Xavier, António V³  Chapman, Stephen K¹  Brennan, Lorraine³  Turner, David L⁴  Salgueiro, Carlos A³ 

[1] Department of Chemistry, University of Edinburgh, West Mains Road, Edinburgh EH9 3JJ, UK;Institute of Cell and Molecular Biology, University of Edinburgh, Mayfield Road, Edinburgh EH9 3JR, UK;Instituto de Tecnologia Quı́mica e Biológica, Universidade Nova de Lisboa, Rua da Quinta Grande 6, 2780-156 Oeiras, Portugal;Department of Chemistry, University of Southampton, Southampton SO17 1BJ, UK

关键词: Multihaem cytochrome;    Nuclear magnetic resonance;    Ring current;    Iron-respiration;    Shewanella;    Sfc;    Shewanella frigidimarina NCIMB400 tetrahaem cytochrome;    Sffcc 3;    Shewanella frigidimarina NCIMB400 flavocytochrome c 3;    Dc 3s;    Desulfovibrio spp cytochromes c 3;    DvHc 3;    Desulfovibrio vulgaris (Hildenborough) cytochrome c 3;    Dgc 3;    Desulfovibrio gigas cytochrome c 3;    NOESY;    nuclear Overhauser spectroscopy;    TOCSY;    total correlation spectroscopy;    COSY;    correlation spectroscopy;   

DOI  :  10.1016/S0014-5793(00)02383-8

学科分类：生物化学/生物物理

来源: John Wiley & Sons Ltd.

【 摘 要 】

The tetrahaem cytochrome isolated during anaerobic growth of Shewanella frigidimarina NCIMB400 is a small protein (86 residues) involved in electron transfer to Fe(III), which can be used as a terminal respiratory oxidant by this bacterium. A 3D solution structure model of the reduced form of the cytochrome has been determined using NMR data in order to determine the relative orientation of the haems. The haem core architecture of S. frigidimarina tetrahaem cytochrome differs from that found in all small tetrahaem cytochromes c ₃ so far isolated from strict anaerobes, but has some similarity to the N-terminal cytochrome domain of flavocytochrome c ₃ isolated from the same bacterium. NMR signals obtained for the four haems of S. frigidimarina tetrahaem cytochrome at all stages of oxidation were cross-assigned to the solution structure using the complete network of chemical exchange connectivities. Thus, the order in which each haem in the structure becomes oxidised was determined.

【 授权许可】

Unknown