Investigating lipid corona formation onto polystyrene nanoparticles through fluorescence correlation spectroscopy

nano-bio interactions;fluorescence correlation spectroscopy;lipid corona

Jacob, Lisa ; Murphy ; Catherine J.

关键词: nano-bio interactions;    fluorescence correlation spectroscopy;    lipid corona;   

Others  :  https://www.ideals.illinois.edu/bitstream/handle/2142/89156/JACOB-THESIS-2015.pdf?sequence=1&isAllowed=y

美国|英语

来源: The Illinois Digital Environment for Access to Learning and Scholarship

【 摘 要 】

When a nanoparticle enters a biological environment, molecules are known to adsorb on the surface forming a corona. Systematically studying the formation of a corona is important to develop knowledge as to how a given nanomaterial will transform once entering a biological environment. A common interface met by a nanomaterial in the human body is the cell membrane, which is composed of phospholipid bilayer. Fluorescence correlation spectroscopy (FCS) is a promising tool that can be used to probe nanoparticle-cell interactions. FCS experiments focusing on exposure of lipid vesicles to different polystyrene nanoparticles indicate possible formation of a lipid corona. Further study varying the nanoparticle charge and lipid vesicle fluidity can help elucidate the mechanism of lipid corona formation. Such work can provide insight into understanding the complex nature of the nano-bio interface.