Measuring Tumor Microenvironment pH During Radiotherapy Using a Novel Cerenkov Emission Multispectral Optical Probe Based on Silicon Photomultipliers

Frontiers in Physics

Ibrahim Oraiqat¹  Issam El Naqa²  Roy Clarke³  Alnawaz Rehemtulla⁴  Essam Al-Snayyan⁴  Andrew Calcaterra⁴ 

[1] Department of Machine Learning, H. Lee Moffitt Cancer Center and Research Center, Tampa, FL, United States;Department of Machine Learning, H. Lee Moffitt Cancer Center and Research Center, Tampa, FL, United States;Department of Radiation Oncology, University of Michigan, Ann Arbor, MI, United States;Department of Physics, University of Michigan, Ann Arbor, MI, United States;Endectra, LLC, Ann Arbor, MI, United States;Department of Radiation Oncology, University of Michigan, Ann Arbor, MI, United States;

关键词: Cerenkov Emission;    radiotherapy;    spectroscopy;    molecular imaging;    silicon photomultipliers;    tumor microenvironment;    Warburg effect;    low light detection;   

DOI  :  10.3389/fphy.2021.636001

来源: Frontiers

【 摘 要 】

Cerenkov Emission (CE) multispectral analysis with silicon photomultiplier (SiPM)-based optical probes is a promising tool for online tumor microenvironment interrogation and targeting during radiotherapy delivery. With the extreme sensitivity of SiPMs, deep tissue multispectral CE measurements can be realized in a clinical setting. In this work, we utilize our Cerenkov Emission Multi-spectral Imaging (CMSI) prototype probe to interrogate the spectral components of the CE signal generated during external beam radiotherapy. Our results demonstrated that CMSI enables effective probing of in vitro quantitative changes in the pH of cell media to monitor cancer cell proliferation after various treatment pathways and differentiate between varying treatment resistance cell lines. In addition, the feasibility of using the CMSI probe in vivo was also successfully demonstrated by measuring tumor pH during a pilot mouse study.

【 授权许可】

CC BY