Study of gene transfection enhancement and parameters optimisation using electroporation microchip

Micro & nano letters

article

Yung-Chiang Chung¹  Wei-Jie Liao¹  Yu-Tzu Huang²  Cheng-Yuan Wu¹ 

[1] Department of Mechanical Engineering, Ming Chi University of Technology;School of Medicine, Fu Jen Catholic University

关键词: genetics;    bioelectric phenomena;    kidney;    cellular biophysics;    biochemistry;    potassium ions;    cytoporation buffer;    293T cells;    buffer solution;    hypoosmolar buffer;    transfection rate;    human embryonic kidney 293 cells;    electric fields;    power consumptions;    gene transfection efficiency;    electroporation microchip;    gene transfection enhancement;   

DOI  :  10.1049/mnl.2013.0594

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

Intensive care unit has a high mortality rate where despite best of the efforts by the healthcare staff, some patients will not survive. Autopsy studies have shown that missed diagnosis is not an infrequent occurrence. However, the rates of hospital autopsies are variable. ‘Digital autopsy’ is a recent modality, which can reduce the need for open autopsies, besides providing useful information about the cause of death instantaneously. Resource constraints mean that it is not universally applicable at present. Apart from correct diagnosis, the ‘process of care’ is also vital to successful outcome. ‘Mortality review’ or audit of all deaths in intensive care should look at this aspect, which in turn will help identify areas for individual, team and system improvements.

【 授权许可】

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