ZnSe:Mn/ZnS quantum dots for the detection of microcystin by room temperature phosphorescence immunoassay

Micro & nano letters

article

Jiaqi You¹  Long Ma¹  Yu He¹  Yili Ge¹  Gongwu Song¹  Jiangang Zhou² 

[1] Hubei Collaborative Innovation Center for Advanced Organic Chemical Materials, Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules, College of Chemistry and Chemical Engineering, Hubei University;Hubei Province Key Laboratory of Regional Development and Environment Response

关键词: zinc compounds;    manganese;    II-VI semiconductors;    chemical sensors;    biological techniques;    semiconductor quantum dots;    phosphorescence;    wide band gap semiconductors;    assembling;    regression analysis;    quantum dot quenching;    microcystin detection;    assembling;    QD;    1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide-N-Hydroxysuccinimide chemistry;    monoclonal antimicrocystin-Leucine Arginine antibody;    RTP immunoassay probe;    room temperature phosphorescence immunoassay probe;    antiMC-LR antibody detection;    regression equation;    interference;    autofluorescence;    light scattering;    temperature 293 K to 298 K;    ZnSeMn-ZnS;   

DOI  :  10.1049/mnl.2018.5690

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

This work has assembled ZnSe:Mn/ZnS quantum dots (QDs)-monoclonal anti-microcystin-Leucine Arginine (anti-MC-LR) antibody bioconjugates as a room temperature phosphorescence (RTP) probe using 1-ethyl-3-(3-dimethyllaminopropyl) carbodiimide/N-Hydroxysuccinimide chemistry for detecting MC-LR. Once the specific interactions occurred between these MCs and their antibodies on the surface of ZnSe:Mn/ZnS QDs, the RTP of ZnSe:Mn/ZnS QDs was quenched with the increased concentration of MC-LR. Under the optimised conditions, the linear range of MC-LR was determined to be 0.9–8.8 nM. The linear relationship between ( P 0 – P )/ P 0 and concentration of MC-LR were observed with the regression equation ( P 0 – P )/ P 0 = 0.03908C + 0.0456 with the detection limit of 0.038 nM. The developed RTP immunoassay favoured environmental applications since the interference from autofluorescence and scattering light was greatly eliminated.

【 授权许可】

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