Green synthesis of silver nanoparticles from Lonicera japonica leaf extract and their anti-inflammatory and antibacterial effects

Micro & nano letters

article

Luyun Yan¹  Beibei Qiu¹  Tongtong Li¹  Dandan Wu¹  Jinhua Zhu¹  Dongbao Zhao¹ 

[1] Henan International Joint Laboratory of Medicinal Plants Utilization, College of Chemistry and Chemical Engineering, Henan University;Center for Multi-Omics Research, School of Life Science, Henan University

关键词: antibacterial activity;    biomedical materials;    cellular biophysics;    biochemistry;    nanoparticles;    nanofabrication;    silver;    nanomedicine;    microorganisms;    particle size;    toxicology;    reduction (chemical);    antibacterial effects;    antiinflammatory effect;    green synthesis;    silver nanoparticles;    aqueous Lonicera japonica leaf extraction;    liquid phase chemical reduction method;    spherical particles;    particle size;    5-lipoxygenase;    cytotoxicity;    RAW264.7 mouse macrophages;    Escherichia coli;    Staphlococcus aureus;    agar well diffusion;    nanomedicine;    nanobiomedical applications;    size 10.0 nm to 20.0 nm;    Ag;   

DOI  :  10.1049/mnl.2019.0343

学科分类：计算机科学（综合）

来源: Wiley

【 摘 要 】

A green synthesis of silver nanoparticles (AgNPs) using aqueous Lonicera japonica leaf extract (AgNPs-LLJ) was reported and their anti-inflammatory and antibacterial effects were investigated. In comparison, AgNPs were also synthesised using the liquid phase chemical reduction method (AgNPs-N 2 H 4 ·H 2 O). The synthesised AgNPs were identified using multiple analytical techniques. The results showed that both AgNPs were spherical particles with an average particle size of 20–30 nm for AgNPs-LLJ and 10–20 nm for AgNPs-N 2 H 4 ·H 2 O. The anti-inflammatory ability of AgNPs was shown by the effective inhibition against 5-lipoxygenase with IC 50 values of 5.08 μg/mL for AgNPs-LLJ and 59.12 μg/mL for AgNPs-N 2 H 4 ·H 2 O, indicating that AgNPs-LLJ had much more obvious anti-inflammatory effect. Furthermore, the cytotoxicity research on RAW264.7 mouse macrophages showed that AgNPs-LLJ had no cytotoxicity at the concentration of 40 μg/mL compared with control. Their antibacterial effects on Escherichia coli (EC) and Staphlococcus aureus (SA) were studied by agar well diffusion method. The maximum antibacterial circles of AgNPs-N 2 H 4 ·H 2 O on EC and SA were 15 and 14 mm, respectively, whereas it was 5.0 and 5.0 mm for AgNPs-LLJ, respectively. The authors conclude that AgNPs-LLJs have a potential to be a nanomedicine for nanobiomedical applications.

【 授权许可】

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